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MONITORING DYNAMIC STATE OF WATER IN PLANT TISSUES USING ^1H-NMR

Research Project

Project/Area Number 06660038
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 園芸・造園学
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

IWAYA Mari  KYUSHU UNIV.AGR.ASSOC.PROF., 農学部, 助教授 (60091394)

Co-Investigator(Kenkyū-buntansha) IKEDA Takefumi  FOREST.and FOR., CHIEF LAB.PROD.INST., KANSAI RES.CTR., 森林総合研究所・関西支所, 室長
OKUBO Hiroshi  KYUSHU UNIV.AGR.ASSOC.PROF., 農学部, 助教授 (80150506)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywords^1H-NMR longitudinal relaxation time (T_1) / Dynamic state of water / Dwarfism / Chilling response / Tulip (Tulipa gesneriana) / Pea (Pisum sativum) / Pine wood nematode / プロトンNMR(^1H-NMR) / MRI / 環境ストレス / 球根 / 縦緩和時間(T_1) / 細胞水
Research Abstract

^1H-NMR is a non-destructive method that can be used to study the dynamic state of water in vivo. Our studies applying NMR longitudinal relaxation times (T_1) showed that although the state of water in plant tissues is primarily influenced by tissue water content, the dynamic state of cell water estimated by T_1 are not necessarily correlate with the tissues water contents. T_1 values in plants are various in many kinds of species, tissues, and organs. Also, T_1 responds sensitively on different kinds of environmental stresses such as chilling, heat, dehydration and inoculation with nematode. 1) This study focuses on the potential dwarfism in seeedlings of Pisum sativum (cv. Progress No.9) in relation to T_1 reflecting the motional freedom of water protons. 2) The physical state of water and metabolic activity in tulip bulbs held at low temperatures were observed using ^1H-NMR imaging and TNBT staining. A strong free water signal was observed in scales for chilled bulb, while a weak water signal was recorded in non-chilled bulb scales. Signal intensity and NMR relaxation time for tulip bulbs reflected differences in the degree of water binding and metabolic activity between chilled and non-chilled bulbs. 3) Water relations and histological features of pine wood inoculated with pine wood nematode was studied. Thus, it may be pointed out that the awareness of "the dynamic states of water in cells or tissues" is very important for the accurate understanding of all fields of plant physiology.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Kaku, S. and M. Iwaya-Inoue: "Environmental stress response in plants studied by ^1H-NMRrelaxation time" Cryobiology and Cryotechnology. 40. 16-24 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 池田、戸田、田島: "マツノザイセンチュウ抵抗性の異なるクロマツとアカマツ家系のマツノザイセンチュウ侵入に対する組織学的反応" 日本植物病理学会報. 60. 540-542 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 井上、大久保、本岡、石田、狩野、小泉: "^1H-NMRイメージングによるチューリップ球根の低温充足度の探査" 園芸学会雑誌. 64. 508-509 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 井上、本岡: "^1H-NMRによるマメ科芽生えにおける矮化特性モニタリング" 園芸学会雑誌. 64. 68-69 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ikeda, T. and T. Kiyohara: "Water relations, xylem embolism and histological features of Pinus thunbergii inoculated with virulent or avirulent pine wood nematoda, Bursaphelench xylophilus." J. Expt. Bot.46. 441-449 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kaku, S.and M.Iwaya-Inoue: "Environmental stress response in plants studied by ^1H-NMR relaxation time." Cryobiology and Cryotechnology. 40 (2). 16-24 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ikeda, T., T.Toda and M.Tajima: "Histological responses of different resistant families of Pinus thunbergii and P.densiflora to an invasion of pine wood nematode (in Japanese)" Ann.Phytopath.Soc.Japan. 60 (4). 540-542 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Iwaya-Inoue, M., H.Okubo, K.Motoka, N.Ishida, H.Kano and M.Koizumi: "Monitoring cold requiment in tulip bulbs by ^1H-NMR imaging. (in Japanese)" J.Jap.Soc.Hort.Sci.64 (2). 508-509 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Iwaya-Inoue, M.and K.Motooka: "Monitoring dwarfism for pea seedlings by ^1H-NMR spectroscopy (in Japanese)" J.Jap.Soc.Hort.Sci.64 (2). 68-69 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ikeda, T.and T.Kiyohara.: "Water relations, xylem embolism and histological features of Pinus thunbergii inoculated with virulent or avirulent pine wood nematoda, Bursaphelench xylophilus." J.Expt.Bot.46 (285). 441-449 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Iwaya-Inoue, M.and S.Kaku: "Dynamic state of water in living plant tissues estimated by ^1H-NMR relaxation times" Plant Cell Physiol.35. 41-41 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 井上眞理ほか5名: "^1H-NMRイメージングによるチューリップ球根の低温充足度の探査" 園芸学会雑誌. 64. 508-509 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 井上眞理・本岡圭: "^1H-NMRによるマメ科芽生えにおける矮化特性モニタリング" 園芸学会雑誌. 64. 68-69 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 池田武文ほか2名: "マツノザイセンチュウ抵抗性の異なるクロマツとアカマツ家系のマツノザイチュウ侵入に対する組織学的反応" 日本植物病理学会報. 60. 540-542 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ikeda,T. and T.Kiyohara: "Water relations, xylem embolism and histological features of Pinus thunbergii inoculated with virulent or avirulent pine wood nematoda, Bursaphelenchus xylophilus." J. Expt. Bot.46. 441-449 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kaku,S.and M.Iwaya-Inoue: "Environmental stress response in palants studied by ^1H-NMR relaxation time" Crobiology and Cryotechnology. 40. (No.2)16-24 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Iwaya-Inoue,M.and S.Kaku: "Dynamic state of water in living p;ant tissues estimated by ^1H-NMR relaxation times" Plant Cell Physiol.35. 41-41 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 井上,眞理ほか5名: "^1H-NMRイメージングによるチューリップ球根の低温充足度の探査" 園芸学会誌別冊. (in press). (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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