• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of Molecular Basis for Gene-for-Gene Complementarity in Plant Diseases : Isolation of an Avirulence Gene from Phytophthora megasperma

Research Project

Project/Area Number 06660044
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

YAMAOKA Naoto  Hokkaido Univ., Grad.Sch.Sci.Associate Professor, 大学院・理学研究科, 助教授 (60174588)

Co-Investigator(Kenkyū-buntansha) TAKEUCHI Yoji  Hokkaido Univ., Grad.Sch.Sci.Instructor, 大学院・理学研究科, 助手 (10250416)
吉川 正明  北海道大学, 理学部, 教授 (00046509)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
KeywordsPhytophthora megasperma / gene-for-gene complementarity / avirulence gene / specific elicitor / soybean / receptor / receptor / elicitor / エリシター
Research Abstract

Disease specificity in a particular combination between a given race and a plant cultivar is called as "race-cultivar specificity". This disease specificity is frequently controlled by gene-for-gene complementarity, in which resistance responses occur only when plant cultivars that express a dominant resistance gene are attacked by pathogen races that express a complementary a virulence gene. The interaction between soybean (Glycine max) and the fungal pathogen Phytophthora megasperma f.sp.glycinea is one of the best understood gene-for-gene plant-pathogen systems in which the processes leading to production of the phytoalexin glyceollin have been analyzed. Mycelial walls of the fungus have been shown to be potent elicitors of glyceollin accumulation, and their possible role in the induction of phytoalexin accumulation in infected plants has been suggested. However, neither specific elicitor explaining specificity between a soybean-cultivar and a race of P.megasperma nor avirulence gene of the fungus has been isolated. Thus, we have tried to isolate an avirulence gene of P.megasperma in this study. Unfortunately, we failed to achieve our final object : to isolate an avirulence gene from P.megasperma. However, we could reveal the existence of potential specific elicitor in intercellular fluid from P.megasperma rase1 infected soybean leaves (cv.Harosoy) to explain a specificity between the fungus and the plant. And also, we could show a structural model of how glucan elicitor is originally present in cell walls of P.megasperma and released by host beta-1,3-endoglucanase ; these data have been published in Plant Physiology (1995). Moreover, we could purify a glucan elicitor-binding protein from the membrane fraction of soybean root cells, and isolate its cDNA ; these data have been published in Proc.Natl.Acad.Sci.USA (1997, in press).

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Okinaka et al.: "A Structural Model for the Mechanisms of Elistor Release from Fungal Cell Walls by Plant β-1,3-Endoglucanase." Plant Physiology. 109. 839-845 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Umemoto et al.: "The Structure and Function of a Soybean β-Glucan-Elicitor-Binding Protein." Proc. Natl. Acad. Sci. USA. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okinaka et al.: "A Structural Model for the Mechanisms of Elisitor Release from FungalCell Walls by Plant beta-1,3-Endoglucanase." Plant Physiology. 109. 839-845 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Umemoto et al.: "The Structure and Function of a Soybean beta-Glucan-Elicitor-Binding Protein." Proc.Natl.Acad.Sci.USA. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Umemoto et al.: "The Structure and Function of a Soybean β-Glucan-Elicitor-Binding Protein" Proc. Natl. Acad. Sci. USA. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Okinaka et al.: "A Structural Model for the Mechanisms of Elisitor Release from Fungal Cell Walls by Plant β-1,3-endoglucanase" Plant Physiology. 109. 839-845 (1995)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi