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Biocontrol of Plant Fungal Diseases by Chitinolytic Microorganisms

Research Project

Project/Area Number 06660046
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物保護
Research InstitutionIbaraki University

Principal Investigator

AKUTSU Katsumi  Ibaraki University Agriculture Associate Professor, 農学部, 助教授 (10151002)

Co-Investigator(Kenkyū-buntansha) HIBI Tadaaki  The Tokyo University Agriculture Professor, 農学部, 教授 (50261954)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsBiocontrol / Chitinase / chitinolytic enzyme gene / Serratia marcescens / Alteromonas sp. / Erwinia ananas / Colletotrichum cyclamenae, / Rhizoctonia solani / Botrytis cinerea / シクラメン灰色かび病
Research Abstract

1. Production of biocontrol agents by transfer of a chitinolytic enzyme gene
Marine bacterium, Alteromonas sp.strain 79401 was shown to suppress the condial growth of plant pathogenic fungi. Botrytis cinerea and Botrytis fabae in vitro by meane of its high chitinolytic activity. To use the ability of this bacterium for biocontrol, a DNA fragment (ca.3.5kb) carrying the chitinolytic enzyme gene was cloned into the plasmid p22-k2. The resulting plasmid, chia-p22-k2/L2 (a9) was introduced into Erwinia ananas L2.B.cineres, B.fabae, Colletotrichum cyclamenae and Rhizoctonia solani could not grow in the vicinity of the transformed E.ananas colonies in vitra Conidial germination and hyphal growth were markedly suppressed. Swelling of the conidia and germ tubes and bursting of hyphal tips were observed under a microscope, suggesting that tha chitinolytic enzyme affected the fungal cell walls. This a9 isolate might be useful for the biocontrol agent of plant pathogenic fungi.
2. Biocontrol of cyclamen diseases by Serratia marcescens B2
In biocontrol testing, cyclamen plants were applied with a highly chitinolytic bacterium, Serratia marcescens isolate B2 and after 24 h, challenge-inoculated with conidia of Botrytis cinerea and Colletotrichum cyclamenae, respectively. The isolate B2 suppressed the fungal disease incidences by ca.60% on attached petals and leaves of cyclamen in a greenhouse. The efficacy of isolate B2 against these fungi was nearly equal to that with 200ppm of iprodione. Although the isolate B2 failed to survive on the petals and leaves more than 2 weeks after the application, the populations on the leaf-bud and the petioles near the base of the cyclamen plant, on which the gray mold and the anthracnose regularly developed, increased 10-fold during this period. These results suggest that S.marcescens B2 is a a potential biocontrol agent against cyclamen fungal diseases under greenhause conditions.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] 伊代住浩幸: "海洋細菌Alteromonas sp.のキチン分解酵素遺伝子を導入したErwinia ananasによるBotrytis cinreaの生育抑制" 日本植物病理学会報. 61. 269 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 片岡 信幸: "Serratia marcescens B2のシクラメン栽培土壌での定着性と萎ちょう病に対する防除機構" 日本植物病理学会報. 61. 268-269 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kazuyuki Hirayae: "In Vitro Growth Inhibition of Plant Pathogenic Fungi, Botrytis spp. by E. coli Transformed with a Chitinolytic Enzyme Gene from a Marine Bacterium,Alteromonas sp." Ann. Phytopathol. Soc. Jpa.62. 30-36 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 宇井 伸一: "Serratia marcescens B2株によるシクラメン炭そ病のバイオコントロール" 日本植物病理学会大会. (4月発表予定). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 染谷信孝: "Serratia marcescens B2株によるシクラメン苗立枯れ病のバイオコントロール" 日本植物病理学会大会. (4月発表予定). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 染谷信孝: "Serratia marcescens B2が誘導するシクラメンの菌類病抵抗性" 日本植物病理学会大会. (4月発表予定). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Iyozumi Hiroyuki: "Biocontrol by Serratia mercescens B2 against gray mold of cyclamen" Ann.Phytopath.Soc.Jpn. 60. 332-333 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nobuyuki Kataoka: "Biocontrol by Serratia marcescens B2 against Fusarium wilt of cyclamen" Ann.Phytopath.Soc.Jpn. 60. 743 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nobuyuki Kataoka: "Survial of Serratia marcescens B2 in soil cultivating cyclamen and its biocontrol mechanism ageinst Fusarium wilt" Ann.Phytopath.Soc.Jpn. 61. 268-269 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Iyozumi Hiroyuki: "Growth suppression of Botrytis cinerea by Erwinia ananas transformed with chitinolytic enzyme gene from Alteromonas sp." Ann.Phytopath.Soc.Jpn. 61. 269 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kazuyuki Hirayaei: "In vitro growth inhibition of plant pathogenic fungi, Botrytis spp., by Escherichia coli transformed with a chitinolytic enzyme gene from a marine bacterium, Alteromonas sp.strain 79401" Ann.Phytopath.Soc.Jpn. 62. 30-36 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Iyozumi Hiroyuki: "Bicontrol of cyclamen gray mold (Botrytis cinerea) by Serratia marcescens B2" Plant Disease. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 伊代住浩幸: "海洋細菌Alteromonas sp.のキチン分解酵素遺伝子を導入したErwinia ananas によるBotrytis cinreaの生育抑制" 日本植物病理学会報. 61. 269- (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 片岡信幸: "Serratia marcescens B2のシクラメン栽培土壌での定着性と萎ちょう病に対する防除機構" 日本植物病理学会報. 61. 268-269 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kazuyuki Hirayae: "In Vitro Growth Inhibition of Plant Pathogenic Fungi, Botrytis spp. by E. coli Transformed with a Chitinolytic Enzyme Gene from a Marine Bacterium, Alteromonas sp." Ann. Phytopathol. Soc. Jpa.62(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 宇井伸一: "Serratia marcescens B2株によるシクラメン炭そ病のバイオコントロール" 日本植物病理学会大会. (発表予定)(1996年4月).

    • Related Report
      1995 Annual Research Report
  • [Publications] 染谷信孝: "Serratia marcescens B2株によるシクラメン苗立枯れ病のバイオコントロール" 日本植物病理学会大会発表予定(1996年4月).

    • Related Report
      1995 Annual Research Report
  • [Publications] 染谷信孝: "Serratia marcescens B2が誘導するシクラメンの菌類病抵抗性" 日本植物病理学会大会. (発表予定)(1996年4月).

    • Related Report
      1995 Annual Research Report
  • [Publications] 伊代住浩幸: "Serratia marcescens B2によるシクラメン灰色かび病のバイオコントロール" 日本植物病理学会報. 60. 332-333 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 片岡 信幸: "Serratia marcescens B2によるシクラメン萎ちょう病のバイオコントロール" 日本植物病理学会報. 60. 743- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kazuyuki Hirayae: "In Vitro Growth Inhibition of Plant Pathogenic Fungi,Botrytis spp.by E.coli Transformed with a Chitinolytic Gene from a Marine Bacterium,Alteromonas sp." Ann.Phytopath.Soc.Japan. 61(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 伊代住浩幸: "海洋細菌Alteromonas sp.のキチン分解酵素遺伝子を導入したErwinia ananasによるBotrytis cinereaの生育抑制" 日本植物病理学会大会発表予定(1995年4月).

    • Related Report
      1994 Annual Research Report
  • [Publications] 片岡 信幸: "Serratia marcescens B2のシクラメン栽培土壌での定着性と萎ちょう病に対する防除機構" 日本植物病理学会大会発表予定(1995年4月).

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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