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DEVELOPMENT OF IMMUNOASSAY FOR PESTICIDE RESIDUES IN IMPORTED AGRICULTURAL PRODUCTS USING MONOCLONAL ANTIBODY

Research Project

Project/Area Number 06660052
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionKOBE UNIVERSITY

Principal Investigator

NAKATA Masanobu  KOBE UNIVERSITY・AGRICULTURE・ASSOCIATE PROFESSOR, 農学部, 助教授 (40031190)

Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsimmunoassay / ELISA / monoclonal antibody / pesticide residue analysis / 残留農薬 / カ-バメート系殺虫剤
Research Abstract

The conventional pesticide residue analyzes by instrumental methods using gas chromatography, high performance liquid chromatography and mass spectrometry are accurate, but require time consuming, expensive equipments and skilled technicians.
In this rsearch, we developed the rapid, simple and high sensitive enzyme immunoassay for pesticide residue analyzes in environmental samples and imported agricultural products using monoclonal antibodies.
Monoclonal antibodies against several pesticides were produced and the assay performances for these agrochemicals were optimized. The working ranges of assay were 10-1,000 ng/ml for insecticide aldicarb and methomil, 1-100 ng/ml for aldicarb sulfon and parathion methyl, and 0.5-10 ng/ml for herbicide chlorpropham.
The results of measurement of pesticides in environmental samples (water and soil) and agricultural products (potato, orange, cabbage) spiked with pesticides showed high recovery without cleanup procedure.
This immunoassay for pesticide residue will be applicable to monitoring of residue in water samples and agricultural products, or can be used in conjunction with instrumental methods.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 中田昌伸,大川秀郎: "ELISA法による除草剤の簡易微量測定法" 植調. 30. 145-152 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 中田昌伸,大川秀郎: "モノクローナル抗体を用いた環境負荷化学物質の酵素免疫測定法" 蛋白 核酸 酵素. 41. 74-80 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] MASANOBU NAKATA AND HIDEO OHKAWA: "SIMPLE ASSAY METHOD FOR HERBICIDES BY ELISA" SYOKUTYOU. 30. 145-152 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] MASANOBU NAKATA AND HIDEO OHKAWA: "ENZYME IMMUNOASSAY WITH MONOCLONAL ANTIBODY FOR ENVIRONMENTAL CONTAMINANTS" PROTEIN,NUCLEIC ACID AND ENZYME. 41. 74-80 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 中田昌伸,大川秀郎: "ELISA法による除草剤の簡易微量測定法" 植調. 30. 145-152 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 中田昌伸,大川秀郎: "モノクローナル抗体を用いた環境負荷化学物質の酵素免疫測定法" 蛋白核酸酵素. 41. 74-80 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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