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Study on production and utilization of plant virus specific antibodies using anti-synthetic peptide antibodies

Research Project

Project/Area Number 06660059
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物保護
Research InstitutionTokyo University of Agriculture

Principal Investigator

NATSUAKI Keiko  Tokyo University of Agriculture, Department of International Agricultural Development, Associate Professor, 農学部, 助教授 (80164482)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsAnti peptide antibody / Plant Virus / Serodiagnosis / SPOTs for epitope analysis / Potyviruses / Synthetic peptides / エピトープ解析 / ペプチド合成 / 抗血清作製 / 特異抗体
Research Abstract

SUMMARY OF RESEARCH RESULTS ; Two potyviruses, clover yellow vein virus (CYVV) and bean yellow mosaic virus (BYMV) are biologically and serologically very colse and it is difficult to distinguish them by the use of poly- and monoclonal antibodies. Upon the information of amino acid sequence of coat protein, the epitope analysis by SPOTs system was conducted and 5 epitopes in N terminals of coat protein region for CYVV-NFU were detected.
As one epitope was common in both BYMV and CYVV, anti-peptide antibody was produced using synthetic peptides. The antibody was used in ELISA, Western blot, ISEM and other serological method to detect CYVV and BYMV without non specific reaction.
SPOTs system was also shown to be useful to detect epitopes which are reacted with monoclonal antibodies in the experiment using monoclonal antibody obtained from ATCC.
Production of some anti-peptide antibodies to some epitopes, however, was not successful because only the antisera with low titer were obtained. It showed that peptides obtained from epitopes detected by SPOTs can not always be used as strong antigens or some anti-peptide antibodies are not suitable to use in some serodiagnosis.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 夏秋啓子・夏秋知英: "合成ペプチドによるクローバー葉脈黄化ウイルス外被タンパクN末端領域のエピトープ解析(講要)" 日本植物病理学会報. 60. 381- (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 夏秋啓子・夏秋知英: "クローバー葉脈黄化ウイルスおよびインゲンマメ黄斑モザイクに対する合成ペプチド抗体の作製(講要)" 日本植物病理学会報. 61. 278-279 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 斉藤敬之・夏秋啓子: "インドネシアのタバコに発生したキュウリモザイクウイルス(CMV)I分離株の性状(講要)" 日本植物病理学会報. 61. 602-603 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 安田茂・夏秋啓子: "アルストロメリアモザイクウイルスの性状と抗血清の作製(講要)" 日本植物病理学会報. 61. 603- (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Keiko T.Natsuaki et al: "Epitope analysis on N terminal regi on of coat protein of clover yellow vein virus using synthetic peptides (Abstract)" Annals of the Phytopathological Society of Japan. 60. 381 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Keiko T.Natsuaki et al: "Production of anti-peptide antibody to clover yellow vein virus and beam yellow mosaic virus (Abstract)" Annals of the Phytopathological Society of Japan. 61. 278-279 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Saito and K.T.Natsuaki: "Properties of a cucumber mosaic vivrus isolate obtained from tobacco plants in Indonesia. (Abstract)" Annals of the Phytopathological Society of Japan. 61. 602-603 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Yasuda and K.T.Natsuaki: "Characterization and antisera production of Alstroemeria mosaic potyvirus. (Abstract)" Annals of the Phytopathol ogical Society of Japan. 61. 603 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 夏秋啓子・夏秋知英: "合成ペプチドによるクローバー葉脈黄化ウイルス外被タンパクN末端領域のエピト-フ解析(講要)" 日本植物病理学会報. 60. 381 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 夏秋啓子・夏秋知英: "クローバー葉脈黄化ウイルスおよびインゲンマメ黄斑モザイクウイルスに対する合成ペプチド抗体の作製(講要)" 日本植物病理学会報. 61. 278-279 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 斉藤敬之・夏秋啓子: "インドネシアのタバコに発生したキュウリモザイクウイルス(CMV)1分離株の性状(講要)" 日本植物病理学会報. 61. 602-603 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 安田茂・夏秋啓子: "アルストロメリアモザイクウイルスの性状と抗血清の作製(講要)" 日本植物病理学会報. 61. 603 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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