|Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
This research was carried out to clarify the function of Filippi's gland joining to the anterior portion of the sikgland of a silkworm, Bombyx mori.
1) Morphometry on the duct of Filippi's gland which joinned to the anterior portion of silkgland was carried out. Each size of length, inside and outside diameter, and wall thickness of the duct showed almost constant values in the 5th instar of larva.
2) Gland morphology and morphometry of PNd, nonspinning strain, did not differ from N124, spinning strain of silkworm.
3) Red pigment injected into the middle portion of silkgland was observed in the lumen of duct tube of filippi's gland.
4) Electric microscopic observations revealed very numerous mitochondria and its location around vacuole, highly development of basal lamina, numerous glycogen granules and microtubules, branched nucleus, and numerous vacuoles with microvilli.
5) With the development of 5th instar larva, contents of DNA,RNA,and protein in gland tissue increased. But specific protein to the function of the gland was not appeared.
6) The pH value of gland tissue was 6.0-6.5.
It is seemed that the function of Filippi's gland is water and solute transport between the silkgland and the hemolymph, maybe from the silkgland to the hemolymph.