| Project/Area Number |
06660078
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
MATOH Toru KYOTO UNIV., FACULTY OF AGRICULTURE,ASSOCIATE PROFESSOR, 農学部, 助教授 (50157393)
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| Project Period (FY) |
1994 – 1995
|
| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Boron / Cell wall / Pectic polysaccharide / Antibody / Immunoblotting / Boron-polysaccharide complex / ホウ酸 / ペクチン |
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| Research Abstract |
Antisera against the Boron-polysaccharide complex (BPC) of radish root cell walls were raised using rabbits. The antisera was produced only when the BPC was injected along with methylated-BSA.Using the antisera, immunoblotting for acidic polysaccharides was established employing a nylon membrane. The specificity of the antisera was examined and it was proved that the antisera was specific to the BPC,irrespective of the origin. Using the antisera, immunoelectron microscopy was carried out, and its ubiquitous localization in cell walls, but its concentration in the corner of cell walls was revealed.
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