| Project/Area Number |
06660080
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Okayama University |
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Principal Investigator |
SHIBASAKA Mineo Research Institute for Bioresources, Research Associate, 資源生物科学研究所, 助手 (60226165)
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| Co-Investigator(Kenkyū-buntansha) |
KATSUHARA Maki Research Institute for Bioresources, Research Associate, 資源生物科学研究所, 助手 (00211847)
NAKASHIMA Susumu Research Institute for Bioresources, Associate Professor, 資源生物科学研究所, 助教授 (60033122)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | ATPase / Hordeum vulgare / plasmamembrane / Salicornia virginica / salinity stress / sugar-phosphate |
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| Research Abstract |
This research project was aimed on determining the characteristics of plasmamembrane contributed to salinity tolerance of a halophyte, Salicornia virginica, roots, based on a physiological and biochemical comparison with mesophyte. Though integrity of mesophyte plasmamembranes has been known to be loss under conditions of high concentration of salt, it has not been clear that what changes in the membranes causes the damage. We planed to investigate what changes in the membranes enhanced permeability and how much important metabolites flowed through the membranes.
Treatments of high NaCl conc.solutions diminished absorption of potassium of a mesophyte, Hordeum vulgare. By the contrast, these treatments enhanced that of a halophyte, Salicornia. We found that important metabolites flowed out of barley roots treated by high NaCl conc.solutions and the high energy bonds was lost. Decrement of potassium uptake in barley could be caused by the loss of energy, which was determined by ^<31>P-NMR and a biochemical quantitation. The loss of membrane integrity was detected by taking up a fluorescent substance (propidium iodide). Releasing peripheral proteins from the membranes and decreasing of plasmamembrane ATPase apoprotein were found simultaneously. However, relationship between these phenomenon and increment of membrane permeability has not be clarified.
Because gene expression needs energy, introducing salinity tolerant gene to barley will be a failure without improving plasmamembrane.
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