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cDNA Cloning and Characterization of Gibberellin-Responsive Genes in Photoblastic Lettuce Seeds

Research Project

Project/Area Number 06660130
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

YAMANE Hisakazu  The University of Tokyo Biotechnology Research Center, Associate Professor, 生物生産工学研究センター, 助教授 (80090520)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsLactuca sativa L. / Phytochrome / Gibberellin / cDNA / Differential screning / Abscisic acid / Alcohol dehydrogenase / レタス(lactuca sativa) / Lactuia satica L. / cDNA cloning / アルコールデヒドトゲナーゼ
Research Abstract

Two cDNA clones, cLRG5 and cLRG11, that respond to gibberellin (GA) were isolated from seeds of photoblastic lettuce (Lactuca sativa L.cv.Grand Rapids) by differential screening. Northern Blot analysis indicated that the levels of LRG5 and LRG11 mRNAs were raised to slightly higher levels 10h after the start of GA treatment and the levels were maintained at least for further 8h, while those in the control seeds gradually decreased. Red light irradiation had effects similar to GA treatment. It was also shown that the increase of levels of LRG5 and LRG11 mRNAs caused by GA or red light treatment was not affected by treatment of abscisic acid (10^<-4> M) that inhibited the seed germination completely. The cLRG5 insert encodes a putative polypeptide of 380 amino acids that is highly homologous to alcohol dehydrogenases from several higher plants. With regard to the cLRG11 insert, no homologous gene has been reported. To investigatin of localization of LRG5 and LRG11 mRNAs in germinating Grand Rapid seeds, in situ hybridization with the coresoponding cDNAs are underway.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] T. Toyomasu ら: "cDNA Cloning and Characterization of Gibberellin-Responsive Genes in Photoblastic Lettuce Seeds" Bioscience, Biotechnology, and Biochemistry. 59. 1846-1849 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Toyomasu, T.Yamauchi, H.Yamane, N.Murofushi, and Y.Inoue: "cDNA Cloning and Characterization of Gibberellin-Responsive Genes in Photoblastic Lettuce Seed" Bioscience, Biotechnology, and Biochemistry. 59 (10). 1846-1849 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toyomasu,T.et al.: "cDNACloning and Chracterization of Gibberellin-responsive Genes in Photoblastic Lettuce Seeds" Biosci.Biotech.Biochem. 59. 1846-1849 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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