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A basic research for the molecular of plant defense system and an application for agricultre.

Research Project

Project/Area Number 06660136
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionOsaka University (1995)
Okayama University (1994)

Principal Investigator

KOBAYASHI Akio  Osaka University, Faculty of engineering, Professor, 工学部, 教授 (30115844)

Co-Investigator(Kenkyū-buntansha) FUKUSAKI Eiichiro  Osaka University, Fuculty of engineering, Associate Professor, 工学部, 助教授 (40273594)
KAJIYAMA Shinichiro  Osaka University, Faculty of engineering, Assistant Professor (20243496)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
KeywordsPlant / Resistance / Secondary metabolism / Elicitor / Oligosaccharide / Polysaccharide / Laminaran / 糖類
Research Abstract

Plants quickly respond to pathogenic invasion. As the result, the secondary metabolism is activated. Therefore, we may use the information obtained from this plant-microbe interaction for secondary metabolite production. For this purpose, we are very much interested in what type of stimulating factors are involved in the activation of the metabolism. We investigated the stimulating factors and we attempted to prepare those, since these eliciting factors can be applicable to produce new secondary metabolites in the plant cell system and a large suply is required. The results of our investigation were listed below.
1) The elicitor activities of deacetylated chitin oligomers to legume plants were investigated.
2) A facile method for preparation of deacetylated chitin oligomers using lysozyme was developed
3) Elicitor-active oligosaccharides from laminaran was purified and thier structures were elucidated.
4) A structure-activity relationship study for elicitor-active oligosaccharide from laminaran was performed.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] A. Kobayashi, et. al.: "Partially N-Deacetylated Chitin Fragments are Strong Elicitors for (+)-Pisatin Induction in Epicotyls of Pea." Z. Naturforsch.49c. 302-308 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A. Kobayashi, et. al.: "Partially N-Deacetylated Chitin Flicitor Induces Antimicrobial Flavonoids in Pea Epicotyls." Z. Naturforsch.49c. 811-818 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A. Kobayashi, et. al.: "Partially N-Deacetylated Chitin Oligomers (Pentamer to Heptawar) are Potential Elicitors for (+)-Pisatin Induction in Pea Epicotyls." Z. Naturforsch.50c. 391-397 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A. Kobayashi, et. al.: "Structural Elucidation of An Elicitor-Active Oligosaccharide, LN-3, Prepared from Algal Laminaran." J. Carbohydr. Chem.14(6). 819-832 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A. Kobayashi, et. al.: "A novel method for chemo-enzymatic synthesis of elicitor-active chitosan oligomers and partially N-deacetylated chitin oligowers using N-acylated chitotrises as substrates in a lysozyme-catalyzed transglycosylation veation" Carbohydrate Research.279. 151-160 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A. Kobayashi, et. al.: "A Minmun Essential Structure of LN-3 Elicitor Activity in Been Cotyledons." Z. Naturforsch.51c(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A.Kobayashi, K.Akiyama, K.Kawazu: "Partially N-Deacetylated Chitin Fragments are Strong Elicitors for (+)-Pisatin Induction in Epicotyls of Pea." Z.Naturforsch.49c. 302-308 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Akiyama, K.Kawazu, and A.Kobayashi: "Partially N-Deacetylated Chitin Elicitor Induces Antimicrobial Flavonoids in Pea Epicotyls." Z.Naturforsch.49c. 811-818 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Akiyama, K.Kawazu, A.Kobayashi: "Partially N-Deacetylated Chitin Oligomers (Pentamer to Heptamar) are Potential Elicitors for (+)-Pisatin Induction in Pea Epicotyls." Z.Naturforsch.50c. 391-397 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A.Kobayashi, A.Tai, and K.Kawazu: "Structural Elucidation of An Elicitor-Active Oligosaccharide, LN-3, Prepared from Algal Laminaran." J.Carbohydr.Chem.14(6). 819-832 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Akiyama, K.Kawazu, and A.Kobayashi: "A novel method for chemo-enzymatic synthesis of elicitor-active chitosan oligomers andpartially N-deacetylated chitin oligomers using N-acylated chitotrioses as substrates in a lysozyme-catalyzed transglycosylation reaction system." Carbohydrate Research. 279. 151-160 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A.Tai, E.Osawa, K.Kawazu, and A.Kobayashi: "A Minimun Essential Structure of LN-3 Elicitor Activity in Been Cotyledons" Z.Naturforsch.51c (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Akiyama: "Partially N-Deacetylated Chitin Oligomers (Pentamer to Heptamer) are Potential Elicitors for(+)-Pisatin Induction in Pea Epicotyls." Z.Naturforsh.50C. 391-397 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] A.Kobayashi: "Structural Elucidation of an Elicitor-Active Oligosaccharide,LN-3,Preparedf rom Algal Laminaran." J.Carbohydr.Chem.14. 819-832 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Akiyama: "A Novel Method for Chmo-enzymatic Synthesis of Elicitor-Active Chitosan Oligomers and Partially N-Deacetylated Chitin Oligomers Using N-Acylated" Carbohydr,Res.279. 151-160 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] A.Kobayashi: "Antifungal compounds induced in the dual culture with Phytolacca americana callus and Botrysis fabae" Z.Naturforsch.50c. in printing (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] A.Kobayashi: "Nostodione A,a novel mitotic spindle poison from a blue-green alga Nostoc Commune." Z.Naturforsch.49c. 464-470 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] A.Kobayashi: "A new type of antifungal phenolics produced by plant peroxdase and its possible role in chemical defence system against plant pathogen." Z.Naturforsch.49c. 411-414 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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