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Antioxidative Function of Plant Polyphenols in Mammalian Cells

Research Project

Project/Area Number 06660162
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 食品科学・栄養科学
Research InstitutionUniversity of Shizuoka

Principal Investigator

NAKAYAMA Tsutomu  University of Shizuoka, School of Food and Nutritional Sciences, Associate Professor, 食品栄養科学部, 助教授 (50150199)

Co-Investigator(Kenkyū-buntansha) HASHIMOTO Kei  University of Shizuoka, School of Food and Nutritional Sciences, Research Associ, 食品栄養科学部, 助手 (10237935)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1994: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsplant polyphenols / active oxygens / hydrogen peroxide / cultured cells / antioxidant / curcumin / caffeic acid / SOD
Research Abstract

(1) Cytotoxicity and DNA single-strand breaks caused by H_2O_2 were assessed by a colony formation assay and a DNA precipitation assay, respectively, with Chinese hamster V79 cells. In both assays, caffeic acid ethyl ester showed protective effects. The structure-activity relationship showed that the omicron-dihydroxy structure of caffeic acid ethyl ester was essential for the protective effects.
(2) We assessed inhibitory effects of curcumin and its chemically modified homologues against H_2O_2-induced cytotoxicity toward Chinese hamster long fibroblasts V79 cells with a colony formation assay. Among 4 curcuminoids, dihydroxycurcumin and dihydroxytetrahydrocurcumin suppressed H_2O_2-induced cytotoxicity. On the contrary, neither curcumin nor tetrahydrocurcumin showed any suppressive effects. These results support out empirical rule that omicron-dihydroxy (catechol) moiety is essential for the inhibitory effects.
(3) We established a simple method to quantify hydrogen peroxide (H_2O_2) generated during the aerobic heating process of (+) -catechin solution Using this method, we found that nonenzymatic H_2O_2 formation from catechin depended on pH,temperature, incubation time, and the presence of O_2 in the solution. The formation of oxidezed products of (+) -catechin, which was estimatied by measuring the absorbance of the solution at 430 nm, also depended on these factors. The H_2O_2 formation was inhibited by various kinds of superoxide dismutase (SOD) with almost the same dose dependency. Although the oxidation of (+) -catechin was enhanced by superoxide (O_2-), neither catalase nor H_2O_2 had any effects on the oxidation. These results suggest that O_2-, rather than H_2O_2, participated in the autoxidation of (+) -catechin, which was coupled with the H_2O_2 formation.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Tsutomu Nakayama: "Hydrogen peroxide formation during catechin oxidation is inhibited by superoxide dismutase" Food Sci.Technol.Int.1. 65-69 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsutomu Nakayama: "Inhibitory effects of caffeic acid ethyl ester on H_2O_2-induced cytotoxicity and DNA single-strand breaks in Chinese hamster V79 cells" Biosci.Biotech.Biochem.60. 316-318 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsutomu Nakayama, Yoko Enoki, and Kei Hashimoto: "Hydrogen peroxide formation during catechin oxidation is inhibited by superoxide dismutase" Food, Sci.Technol.Int. 1, (1). 65-69 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsutomu Nakayama, Munetaka Yamada, Toshihiko Osawa, and Shunro Kawakishi18GB02 : Inhibitory effects of caffeic acid ethyl ester on H_2O_2-induced cytotoxicity and DNA single-strand breaks in Chinese hamster V79 cells: Biosci.Biotech.Biochem. 60, (2). 316-318 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Nakayama et al.: "Hydrogen peroxide formation during catechin oxidation is inhibited by SDD." Food Sci.Technol.Int.1. 65-69 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Nakayama et al.: "Inhibitory effects of caffeic acid ethyl ester on H_2O_2-induced cytotoxicity and DNA single-strand breaks in Chinese hamster V79 cells." Biosci.Biotech.Biochem.60. 316-318 (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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