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Vibriosis of kuruma prawn : Detection of the causative bacterium by PCR

Research Project

Project/Area Number 06660238
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General fisheries
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

MUROGA Kiyokuni  Hiroshima Univ.Fac.Appl.Biol.Sci., Professor, 生物生産学部, 教授 (30011993)

Co-Investigator(Kenkyū-buntansha) NAKAI Toshihiro  Hiroshima Univ.Fac.Appl.Biol.Sci., Associate Professor, 生物生産学部, 助教授 (60164117)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsVibrio penaeicida / PCR (RT-PCR) / Kuruma prawn / Vibriosis / detection / rRNA / primer / ビブリオ菌 / rRNA / PCR / 塩基配列
Research Abstract

Taxonomical position of the causative bacterium of vibriosis in kuruma prawn, Vibrio sp.PJ was established and a detection methood for the pathogen by PCR method was developed.
1. Vibrio penaeicida n.sp.was proposed as the name of the pathogen.
2. It was found that the pathogen multiplied in the stomach, midgut gland and lymphoid organs of experimentally infected kuruma prawns, but a detection method with higher sensitivity was needed for the determination of the initial multiplication site.
3. Based on the base sequence data of Vibrio 16SrRNA,species-specific sequence was determined by cloning.
4. Based on the above specific sequence, a set of primers was designed for RT-PCR.The aimed product was obtained from all strains of V.penaeicida but not from other vibrios.
5.It was found that GITC-chlorophorm or Tween20-phenol method was applicable for the extraction of RNA from kuruma prawn.
6. The above RT-PCR method gave higher detectin rate of V.penaeicida from carrier kuruma prawns than conventional culture method.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] K.Ishimaru: "Vibrio penaecida sp.nov,a pathogen of Kuruma prawns(Penaeus japonicus)" International Journal of Systematic Bacteriology. 45. 134-138 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] L.D.de la Pena: "Dynamics of Vibrio sp.PJ in organs of orally infected kuruma prawn" Fish Pathology. 30. 39-45 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Genmoto: "16SrRNA targeted RT-PCR for the detection of Vibrio penaeicida,the pathogen of cultured kuruma prawn,Penaeus japonicus" Diseases of Aquatic Organisms. 23(印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Ishimaru: "Vibrio penaecida sp.nov, a pathogen of kuruma prawns (Penaeus joponicus)" International Journal of Systematic Bacteriology. 45. 134-138 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] L.D.de la Pena: "Dynamics of Vibrio sp.PJ in organs of orally infected Kuruma prawn" Fish Pathology. 30. 39-45 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Genmoto: "16SrRNA targeted RT-PCR for the detection of Vibrio penaeicida, the pathogen of cultured Kuruma prawn, Penaeus japonicus" Diseases of Aguatic Organisms. 23 (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Genmoto: "16S rRNA targeted RT-PCR for the detection of Vibrio penaeicida,the pathogen of cultured kuruma prawn Penaeus japonicus" Diseases of Aquatic Organisms. 23(印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Ishimaru: "Vibrio penaeicida sp.nov.,a pathogen of kuruma prawns(Penaeusjaponicus)" Internal Journal of Systematic Bacteriology. 45. 134-138 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] L.D.de la Pena: "Dynamics of Vibrio sp.PJ in organs of orally infected kurama prawn" Fish Pathlogy. 30. 39-45 (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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