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RAPD analysis of white clover cultivars and clones for swards with intensive grazing and those with low-input

Research Project

Project/Area Number 06660337
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Zootechnical science/Grassland science
Research InstitutionShizuoka University

Principal Investigator

SAWADA Hitoshi  Shizuoka University Faculty of Agriculture, Associate Professor, 農学部, 助教授 (10183831)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsWhite clover / Clone identification / RAPD markers / Cultivar identification / Plant population ecology / Grassland ecology / Pasture-plant breeding / Clonal plant / 放牧育種 / 牧草草地 / 品種識別法
Research Abstract

The aims of this research are (1) to develop a technique for identifying cultivars and clones of white clover using and (2) to examine the advantageous architecure in grazed swards. The technique and information are much useful in understanding the coexistence strategy of white clover inhabiting intensively grazed swards and sustainable swards. Firstly, I examined the possibility of identifying two cultivars (large-leaved cv. Kopu and small-leaved cv. Tahora) and their clones using RAPD markers. A screen-test showed that ten primers were useful and the combination of the performance from the primers gave well results in the identification. Use of both RAPD and isozyme markers provides well identification with relatively low cost.Secondly, I assessed the advantageous architecture of white clover in two grazed swards (zoysia-typed and ryegrass-typed). They are considered as a typical system for sustainable use and for intensive use, respectively. I used the studying approach combined (1) detailed observations and measurements in mini-swards experimentally set up and (2) observations in fields. Obserations and measurements in a ryegrass-sward showed that large-leaved type showed larger survival and growth performance, whereas the revearse was true in intensive swards. They also suggested that differences in both attributes between two types may be small in intensive swards due to larger plasticity of larger-leaved type. For a zoysia-sward, a following hypothesis was tested : If a newly forming patch involves a large-leaved clone, it grows rapidly to occupy the patch area by the end of the patch longevity. Results of the mini-sward experiment and observations in a field supported this hypothesis strongly. They also suggested that climatic conditions affected this trend strongly.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Hitoshi Sawada: "Variation in seed reproductive characters within the seed population of white clover(Trifolium repens L.) in a Trifolium repens-Zoysia japonica coexisting sward." Grassland Science. 41. 1-8 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hitoshi Sawada: "Pollinator behavior in white clover(Trifolium repens L.)patches and its consequences to seed set in a Zoysia-type sward." J.Jpn.Grassl.Sci.40. 448-456 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 萩ノ迫 善六: "富士山麓の異なる標高に自生するシロクローバ(Trifolium repens L.)集団間の外部形態,バイオマス分配,クローナル成長パターンの変異." 日本草地学会誌. 41. 319-324 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hitoshi Sawada: "Variation in seed reproductive characters within the seed population of white clover (Trifolium repens L.) in a Trifolium repens-Zoysia japonica coexisting sward." Grassland Science. 41 (1). 1-8 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hitoshi Sawada: "Pollinator behavior in white clover (Trifolium repens L.) patches and its consequences to seed set in a Zoysia-type sward." J.Jpn.Grassl.Sci.40 (4). 448-456 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Zenroku Oginosako: "Phenotypic variations in plant architecture, biomass allocation and clonal growth pattern among white clover (Trifolium repens L.) populations inhabiting different altitudes of Mt.Fuji.(In Japanese)" Grassland Science. 41 (4). 319-324 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hitoshi Sawada: "Variation in seed reproductive characters within the seed population of white clover(Trifolium repens L.)in a Trifolium repens-Zoysia japonica coexisting sward." Grassland Science. 41. 1-8 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hitoshi Sawada: "Pollinator behavior in white clover(Trifolium repens L.)patches and its consequences to seed set in Zoysia-type sward." J. Jpn. Grassl. Sci.40. 448-456 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 荻の迫 善六: "富士山麓の異なる標高に自生するシロクローバ(Trifolium repens L.)集団間の外部形態,バイオマス分配,クローナル成長パターンの変異." 日本草地学会誌. 41. 319-324 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hitoshi Sawada: "Outcome and processes of competition between white clover (Trifolium repens)cultivars exhibiting different architecture:consequences of phenotypic plasticity and intra-population variation"

    • Related Report
      1994 Annual Research Report
  • [Publications] Hitoshi Sawada: "Outcome and processes of compectition between white clover (Trifolium repens)cultivars exhibiting different architecture:pasture experiments"

    • Related Report
      1994 Annual Research Report
  • [Publications] Hitoshi Sawada: "Within-patch dynamics of white clover(Trifolium repens)clones exhibiting different architecture:A direct observation"

    • Related Report
      1994 Annual Research Report
  • [Publications] Hitoshi Sawada: "Random amplified polymorphic DNA analysis of Whit clover(Trifolium repens)cultivars"

    • Related Report
      1994 Annual Research Report
  • [Publications] Hitoshi Sawada: "Implications of architectural variations of white clover(Trifolium repens)to inter specific competition with Zoysia japonica"

    • Related Report
      1994 Annual Research Report
  • [Publications] Hitoshi Sawada: "Physiological integration of white clover(Trifolium repens)cultivars exhibiting different architecture"

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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