| Project/Area Number |
06660351
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied animal science
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| Research Institution | Shinshu University |
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Principal Investigator |
ONO Tamao Shinshu University, Agriculture, Associate Professor, 農学部, 助教授 (10177264)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAGI Yuji Shinshu University, Agriculture, Assistant Professor, 農学部, 助手 (20226757)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Quail / Chicken / Embryonic development / Transgenesis / Primordial germ cells / Blastoderm / Embryo culture / gonad |
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| Research Abstract |
Production of offspring derived from transferred foreign primordial germ cells (PGCs) will be highly attractive strategy for genetic improvement of domestic animals including poultry. In this study we transfused circulating PGCs of quail embryos to chick embryos and vice versa. Treated embryos were cultured in vitro in surrogate chicken egg-shells until reached to stage 29 of H&H and then observed the population of foreign PGCs settled in the embryonic gonads of host animals. The endogenous and exogenous PGCs were immunohistochemically stained with chick PGCs-positive 2C9 and quail PGCs-positive QCR1 monoclonal antibodies. For double immunohistochemistry paraffin sectioned embryonic gonads were treated with 2C9, visualized with DAB,removed the antibody from the antigen with 0.1M glycine-HC1 (pH2.2) , treated with QCR1 and visualized with 1-cloro-4-naphthol. Transferred quail PGCs were settled in the gonads of all the surviving chick embryos (5/10) and shared 3-19% of total population of PGCs in the gonad. In the reverse case, transfer of chick PGCs to quail embryos, chick PGCs were also settled in the gonads of all the surviving embryos (8/10) and shared 1-10% of of total population of PGCs.
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