| Project/Area Number |
06660378
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
ISHII Takashi KYOTO UNIV., AGRICULTURE,INSTRUCTOR, 農学部, 助手 (70111945)
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| Project Period (FY) |
1994 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥300,000 (Direct Cost: ¥300,000)
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| Keywords | PITUITARY / IMMUNOHISTOCHEMISTRY / IMAGE ANALYSIS / 画像解析 |
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| Research Abstract |
In sheep anteri or pituitary, author examined simultaneous identification of two or more types of immunolabelled cells and analyzed a morphological relationship between different neighboring cell types in addition to the size, number and their proportions of each cell type using an image analysis system. The image analysis system could give different pseudocolors to different types of immunolabelled cells and make a montage from the two images of the respective types of cells. The findings were that the cross-sectional area of TSH immunolabelled cell was significantly larger (p<0.05) in the caudal measuring field than in the rostral one. There were no significant differences between the rostral and caudal fields in those of PRL-, GH- and LHbeta-immunolabelled cells. The proportions of number and area of immunolabelled cells was the largest in the PRL cells, followed by the GH cells. Those of the ACTH,TSH and LHbeta cells were the almost same and low percentage. The neighboring ratio--the average number of a cell type adjoining the other cell types were inferred in the following combination of immunolabelled cells : ACTH to PRL as 3 : 2 ; ACTH to TSH as 1 : 1 ; PRL to TSH as 1 : 2 ; PRL to GH as 3 : 4 ; TSH to GH as 3 : 2 ; TSH to LHbeta as 1 : 1 ; and GH to LHbeta as 2 : 3. The present method would be effective for morphometry of immunolabelled cells in sheep pituitary using the image analysis system.
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