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DETERMINATION OF FETAL SEX BY ULTRASONOGRAPHY AND PCR USING FETAL FLUID ASPIRATED BY AMNIOCENTESIS IN COWS

Research Project

Project/Area Number 06660404
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Applied veterinary science
Research InstitutionKAGOSHIMA UNIVERSITY

Principal Investigator

KAMIMURA Shunichi  ASSOCIATE PROFESSOR,DEPARTMENT OF VETERINARY MEDICINE,FACULTY OF AGRICULTURE,KAGOSHIMA UNIVERSITY, 農学部, 助教授 (90233949)

Co-Investigator(Kenkyū-buntansha) HAMANA Katsumi  PROFESSOR,DEPARTMENT OF VETERINARY MEDICINE,FACULTY OF AGRICULTURE,KAGOSHIMA UNI, 農学部, 教授 (30011977)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsamniocentesis, / cow, / fetal sex, / PCR, / ultrasound
Research Abstract

Sex of the bovine fetus was predicted by ultrasonography and Y-chromosome specific DNA amplified by PCR in fetal fluid, which was aspirated by transvaginal-transuterine ultrasound-guided amniocentesis. Ultrasound examination were done in 68 cows through 117 trials between Day 35 to 93. Sex was correctly diagnosed as male in 43/46 (95.3%) and as female in 40/44 (90.9%) . Sex was not diagnosed in 27 cows. A total of 35 aspirates from 30 cows, 15 Holsteins and 15 Japanese Blacks at from 59 to 250 days of pregnancy were used. Five cows were aspirated twice at a 10 day interval. A 5.0 MHz convex array transducer connected to a scanner was inserted into the vagina under caudal epidural anesthesia. The transducer was equipped with a 65cm long, 21 gauge needle within the probe carrier. A bovine male-specific primer and a bovine gender-neutral primer were used. Fetal fluid was obtained from all except two cows in early pregnancy. Five animals aborted within one week following aspiration. A total of 33 samples, 29 of amniotic fluid and 4 of allantoic fluid, were subjected to PCR analysis. Fetal gender was identified in 31/33 samples (18 females and 13 males) . Gender was also determined by gross examination of external genitalia of offspring after calving or abortion. Fetal gender was correctly identified by PCR analysis of aspirated fetal fluid in 16/16 females and in 13/15 males. Transvaginal ultrasound-guided amniocentesis followed by PCR analysis of aspirated cell DNA can be used to accurately determine fetal sex in cows at from 70 to 100 days of pregnancy. The procedure requires considerable skill and is not without some risk to fetal viability.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Kamimura S.: "Determining sex of the bovine fetus with Y-chromosome specific DNA amplified by PCR in fetal fluid aspirated by transvaginal-transuterine ultrasound-guided amniocentesis." Proc. 18th World Buiatrics Congress.1. 265-268 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 西山公二、他: "超音波ガイド法による卵子および羊水の経膣穿刺" 鹿児島県獣医師会会報. 13. 23-26 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 上村俊一、他: "黒毛和種受胚牛の超音波断層装置による卵巣所見と血液中progesterone濃度" 鹿大農学術報告. 44. 29-34 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 多久津昌治、他: "PCR法によるウシの初期胚と胎子の性判定" 西日本畜産学会報. 37. 43-47 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kamimura S.: "Turrover of ovulatory and non-ovulatory dominant follicles in postpartum Japanese black cows." J. Reprod. Dev.40. 171-176 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kamimura S.: "Ultrasonic diagnosis of bovine fetal sex by the relative location of the genital tubercle in early pregnancy." J. Reprod. Dev.40. 343-347 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kamimura,S.: "Determination of fetal sex by PCR using fetal fluid aspirated by transvaginal ultrasound-guided amniocentesis in cows" Aust. Socie. Reprod. Biol.27. 70-70 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 上村俊一、他: "分娩後の牛の卵胞形成に及ぼすcarry-over effectの影響" 鹿大農学術報告. 45. 59-63 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 上村俊一: "牛の臨床繁殖における超音波診断法" 臨床獣医. 13. 20-28 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kamimura,S.: "Determination of fetal sex by PCR using fetal fluid aspirated by transvaginal ultrasound-guide amniocentesis in cows" Theriogenology. (Submitted). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kamimura S., Nishiyama K., Sameshima H., Hamana K.: "Determining sex of the bovine fetus with Y-chromosome specific DNA amplified by PCR in fetal aspirated by transvaginal-transuterine ultrasoud-guided amniocentesis." Proc.18th World Buiatrics Congress. 1. 265-268 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nishiyama K., Kamimura S., Nagano M., Goto K., Hamana K.: "Ultrasound-guided transvaginal aspiration of follicular oocyte and amniotic fluid." J.Kagoshima Vet.Med.Assoc.13. 23-26 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kamimura S., Ono M., Sameshima H., Enomoto S., Hamana K.: "Ultrasonic findings in the ovaries and associated concentrations of plasma progesterone in Japanese black cows transferred with frozen-thawed embryos." Bull.Fac.Agri.Kagoshima Univ.44. 29-34 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ookutsu S., Maeda Y., Goto K., Kamimura S., Yanagida K., Nakanishi K., Kuboto C., Kajisa O., Kawabata K., Yokoyama K., Inohae S.: "Sexing of bovine embryos and fetuses by the polymerase chain reaction technique." Western Japan.J.Animal Science.37. 43-37 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kamimura S., Sameshima H., Enomoto S., Hamana K.: "Turnover of ovulatory and non-ovulatory dominant follicles in postpartum Japanese black cows." J.Reprod.Dev.40. 171-176 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kamimura S., Kondoh S., Enomoto S., Sameshima H., Goto K., Hamana K.: "Ultrasonic diagnosis of bovine fetal sex by the relative location of the genital tubercle in early pregnancy." J.Reprod.Dev.40. 343-347 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kamimura S., Mizushima Y., Nagano M., Hamana K.: "Previous pregnancy carry-over effects on the folliculogenesis in the postpartum cows ovaries." Bull.Fac.Agri.Kagoshima Univ.45. 59-63 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kamimura S., Ookutsu S., Goto K., Hamana K.: "Determination of fetal sex by PCR using fetal fluid aspirated by transvaginal ultrasound-guided amniocentesis in cows." Proc.27th Australian Society for Reproductive Biology. 71. (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kamimura S.: "Ultrasonography in clinical reproduction in cows." Clinical Veterinary Practice.13 (10). 20-28 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kamimura S., Nishiyama N., Ookutsu S., Goto K., Hamana K.: "Determination of fetal sex by PCR using fetal fluid aspirated by transvaginal ultrasound-guide amniocentesis in cows." Theriogenology. (Submitted). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kamimura,S.: "Determination of fetal sex by PCR using fetal fluid aspirated by transvaginal ultrasound-guided amniocentesis in cows" Aust.Socie.Reprod.Biol.27. 70-70 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 上村俊一、他: "分娩後の牛の卵胞形成に及ぼすcarry-over effectの影響" 鹿大農学術報告. 45. 59-63 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 上村俊一: "牛の臨床繁殖における超音波診断法" 臨床獣医. 13. 20-28 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kamimura,S.: "Ultrasonic Diagnosis of Bovine Fetal Sex by the Relative Location of the Genital Tubercle in Early Pregnancy" J.Reprod.Dev.40. 343-347 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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