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Molecular and cellular biology of dynamics and mechanisms of myofibrillogenesis

Research Project

Project/Area Number 06670010
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionChiba University

Principal Investigator

SHIMADA Yutaka  Chiba University School of Medicine Professor, 医学部, 教授 (70009116)

Co-Investigator(Kenkyū-buntansha) KOMIYAMA Masatoshi  Chiba University School of Medicine Assistant, 医学部, 助手 (70175339)
TOYOTA Naoji  Chiba University School of Medicine Lecturer, 医学部, 講師 (00188822)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
Keywordsmyofibril / myoprotein / actin / myosin / troponin / development / regeneration / dynamics / アイソフォーム / 筋形成因子 / in situ hybridization / 螢光抗体法 / 筋原線維形成 / コネクチン / ネブリン / 分子種
Research Abstract

1.Order of appearance of myofibrillar proteins in embryonic chicken somites : Immunofluorescence microscopy revealed that in chicken cervical somites the proteins constituting myofibrils are not synthesized simultaneously and the turning-on of the gene expression of the adult isoforms was not dependent on the completion of the appearance of the major myofibrillar proteins. In situ hybridization showed that cardiac type troponin C mRNA appeared one stage (about 3 hours) earlier than its protein.
2.Troponin and dystrophin in regenerating muscle : In regenerating fibers of the slow (anterior latissimus dorsi) and fast (posterior latissimus dorsi) muscles of adult chickens, embryonic troponin was expressed earlier than dystrophin and changed to its adult isoform later than the stage when dystrophin exhibited the same appearance as that in normal muscle fibers. Further, although regenerating fibers in denervated muscle switched on the expression of troponin similarly to those in innervated m … More uscle, many of them again reverted to an embryonic state. However, no difference was found in the dystrophin expression between regenerating fibers with nerves intact and those with nerves resected. Thus, it appears that the expression of troponin and dystrophin genes in regulated independently during regeneration and nerves are required for the full development of the former protein while unnecessary for the latter.
3.Protein dynamics during myofibrillogenesis : Immunogold electron microscopy of cardiomyocytes microinjected with biotin-actin and -myosin showed that gold labeling was first found at the A band and the A-I junctional level of proximal myofibrils, respectively. Thus, polymerization of actin and myosin seems to occur in association with myosin filaments and with A-I junctional regions of myofibrils, respectively. Fluorescence recovery (FR) study of rhodamine-labeled muscle and nonmuscle actins microinjected into cultured chicken cardiomyocytes and fibroblasts showed that stress fibers had faster FR than myofibrils ; in myofibrils FR of muscle actin was faster than that of nonmuscle actin, and nascent myofibrils displayd different FR ; and stress fibers had similar FR at both proximal and terminal portions with both muscle and nonmuscle actins. Less

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Masatoshi Komiyama: "Troponin and dystrophin in regenerating muscle fibers with and without denervation." Muscle & Nerve. 17(9). 1062-1064 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Zhen-Hua Zhou: "Effects of cycloclorotine on myofibrils in cardiomyocytes and on actin filament bundles in fibroblasts in vitro." Natural Toxins. 2(6). 378-385 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yutaka Shimada: "Development of connection/titin and nebulin in striated muscles of the chicken." Advances in Biophysics. 33. 223-234 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Masatoshi Komiyama: "The intracellular sorting of myosin alkali light chain isoproteins reflects the sequence of developmental expression as determined by double epitope-tagging competition." Journal of Cell Science. 109. 2089-2099 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yutaka Shimada: "Dynamics of actin in cardiac myofibrils and fibroblast stress fibers." Cell Structure and Function. 22(1)(印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Naoji Toyota: "Developmental Mechanisms of Heart Diseases (Expression of troponin C and I genes analyzed at the mRNA and protein levels in developing muscles.)" Futura., 679(71-77) (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 嶋田 裕: "新筋病理学(デスミン,ビメンチン,シネミン,フィラミン)" 南江堂, 946(254-260) (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Komiyama, M., Toyota, N.and Shimada, Y.: "Troponin and dystrophin in regenerating muscle ribers with and without denervation" Muscle Nerve. 17 (9). 1062-1064 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Zhou, Z.-H., Komiyama, M., Terao, K.and Shimada, Y.: "Effects of cycloclorotine on myofibrils in cardiomyocytes and on actin filament bundles in fibroblasts in vitro" Natural Toxins. 2 (6). 378-385 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shimada, Y., Komiyama, M., Begum, S.and Maruyama, K.: "Development of connectin/titin and nebulin in striated muscles of chicken" Adv.Biophys.33. 223-234 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Komiyama, M., Soldati, T., von Ary, P.and P Perriard, J.-C.: "The intracellular sorting of myosin alkali light chain isoproteins reflects the sequence of developmental expression as determined by double epitope-tagging competition" J.Cell Sci.109. 2089-2099 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shimada, Y., Suzuki, H.and Konno, A.: "Dynamics of actin in cardiac myofibrils and fibroblast stress fibers" Cell Struct.Funct.22 (1) (In press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Toyota, N.and Shimada, Y.: Expression of troponin C and I genes analyzed at the mRNA and protein levels in developing muscles. In : Developmental Mechanisms of Heart Diseases. Futura, 71-77 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Masatoshi Komiyama: "Troponin and dystrophin in regenerating muscle fibers with and without denervation." Muscle & Nerve. 17(9). 1062-1064 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Zhen-Hua Zhou: "Effects of cycloclorotine on myofibrils in cardiomyocytes and on actin filament bundles in fibroblasts in vitro." Natural Toxins. 2(6). 378-385 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yutaka Shimada: "Development of connectin/titin and nebulin in striated muscles of the chicken." Advances in Biophysics. 33. 223-234 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masatoshi Komiyama: "The intracellular sorting of myosin alkali light chain isoproteins reflects the sequence of developmental expression as determined by double epitope-tagging competition." Journal of Cell Science. 109. 2089-2099 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yutaka Shimada: "Dynamics of actin in cardiac myofibrils and fibroblast stress fibers." Cell Structure and Function. 22(1)(印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Naoji Toyota: "Developmental Mechanisms of Heart Diseases (Expression of troponin C and I genes analyzed at the mRNA and protein levels in developing muscles.)" Futura, 679(71-77) (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 嶋田裕: "新筋病理学(デスミン,ビメンチン,シネミン,フィラミン)" 南江堂, 946(254-260) (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yutaka Shimada: "Development of connectin/titin and nebulin in striated muscles of chicken." Advances in Biophysics. (印刷中).

    • Related Report
      1995 Annual Research Report
  • [Publications] Masatoshi Komiyama: "Troponin and dystrophin in regenerating muscle fibers with and without denervation." Muscle & Nerve. 17. 1062-1064 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masatoshi Komiyama: "Dynamics of actin and assembly of connectin (titin) during myofibrillogenesis in embryonic chick cardiac muscle cells in vitro." Developmental Dynamics. 196. 291-299 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fujio Atsuta: "Distribution of connectin (titin), nebulin and α‐actinin at myotendinous junctions of chicken pectoralis muscles: an immunofluorescence and immunoelectron microscopic study." Journal of Muscle Research and Cell Motility. 14. 511-517 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] Katsunori.Kouchi: "Incorporation of microinjected biotin‐labelled actin into nascent myofibrils of cardiac myocytes: an immunoelectron microscopic study." Journal of Muscle Research and Cell Motility. 14. 292-301 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] Naoji Toyota: "Expression of troponin C genes during development in the chicken." International Journal of Developmental Biology. 37. 531-537 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] Naoji Toyota: "Developmental Cardiology. Inborn Heart Diseases: Developmental Mechanisms" Futura, N. Y., 679(71‐77) (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masatoshi Komiyama: "Troponin and dystrophin in regenerating muscle fibers with and without denervation." Muscle & Nerve. 17. 1062-1064 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Masatoshi Komiyama: "Dynamics of actin and assembly of connectin (titin) during myofibrillogenesis in embryonic chick cardiac muscle cells in vitro." Developmental Dynamics. 196. 291-299 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Fujio Atsuta: "Distribution of connectin (titin),nebulin and α-actinin at myotendinous junctions of chicken pectoralis muscles: an immunofluorescence and immunoelectron microscopic study." Journal of Muscle Research and Cell Motility. 14. 511-517 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Katsunori Kouchi: "Incorporation of microinjected biotin-labelled actin into nascent myofibrils of cardiac myocytes: an immunoelectron microscopic study." Journal of Muscle Research and Cell Motility. 14. 292-301 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yutaka Shimada: "Distribution of connectin (titin) and transverse-tubules at myotendinous junctions." Scanning Microscopy. 7. 157-163 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Naoji Toyota: "Expression of troponin C genes during development in the chicken." International Journal of Developmental Biology. 37. 531-537 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Naoji Toyota: "Developmental Cardiology. Inborn Heart Diseases: Developmental Mechanisms" Futura,N.Y.(印刷中),

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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