| Project/Area Number |
06670012
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY |
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Principal Investigator |
OHTANI Osamu Toyama Medical and Pharmaceutical University Medicine, Anatomy, Professor, 医学部, 教授 (90127548)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | neovascularization / new formasltion of lymphatics / mesentery / immunohistochemistry / ultrastructure / confocal laser scanning microscopy / scanning electron microscopy / rat |
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| Research Abstract |
To elucidate the mechanisms of new formation of blood and lymphatic vessels is quite important in developing the therapeutic methods for abnormal angiogenic and ischemic diseases. This study aimed to reveal morphological mechanisms of neovascularization induced by compound 48/80 in the rat mesentery.
Blood capillaries developed as sprouts from the venules or capillaries. The pericytes surrounded the basal surfaces of newly formed blood vessels. Some pericytes extended their processes into the surrounding interstitial tissue space. There were desmin-positive, pericyte-like cells near the growing vessels. The pericytes surrounding the capillaries showed characteristic shapes, but as the vessels became larger, the pericytes gradually changed their shapes into smooth muscle ones. These suggested that fibroblasts became pericytes which in turn changed into smooth muscle cells. In addition, the pericytes appeared to guide the endothelial cells of developing vessels.
Lymphatic vessels also developed by sprouts from the pre-existing vessels. There were, however, some newly formed lymphatics free from the pre-existing ones. Blood capillary sprouts with pericytes were frequently in touch with developing lymphatics. This suggested that capillary sprouts and/or their pericytes might play a role in keeping a certain spatial relationship between blood and lymphatic vessels, or in guiding new formation of lymphatics.
Further study will be needed to elucidate the mechanisms by which blood and lymphatic vessels are newly formed in normal and pathological conditions.
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