| Project/Area Number |
06670037
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Keio University |
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Principal Investigator |
AISO Sadakazu Keio Univ.School of Med.Dept.of Anat., Professor, 医学部, 教授 (60138013)
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| Co-Investigator(Kenkyū-buntansha) |
OGAWA Motoyuki Keio Univ.School of Med.Dept.of Anat.Fellow, 医学部, 助手 (90255422)
SHIOZAWA Masahide Keio Univ.School of Med.Dept.of Assist.Professor, 医学部, 講師 (50170840)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | transugenic mouse / HLA-DQ / Immunoregulation |
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| Research Abstract |
In mammals Major Histocompatibility Complex class II molecules are expressed on B cells, antigen presenting cells ant thymic epithelial cells, and they are responsible for constructing immune repertoir in the body. In humans, HLA-DQ is one of the MHC class II genes and it was reported to be relevant to insuline-dependent diabetes mellitus (IDDM). In this project, we are making transgenic B10.S X SJL mice with DQ transgenes which have positive and negative association with IDDM.
In the past two year, the construct DNAs from DQW6a and b genomic clones were comicroinjected into the fertilized eggs of B10.S X SJL mice. The DQW8alpha and beta genomic clones were also co-microinjected into the fertilized eggs of B10.S X SJL mice to produce DQW8 transgenic mice The concentration of DNA solution was about 150 copies of ech DNA insert per pico-liter, presumably per injicted egg. Out of pups born, four mice were shown to have transgenes. By flow cytometry, the DQ positive cells cells were shown to be la-positive cells.
The examination of DQ expression and immune responses in those mice will help define DQ-restricted antigen-specific immune responses as well as the role of specific DQ amino acid sequences in autoimmune responses in IDDM.
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