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Physiological effect of vasoactive peptides and its receptors on metabolism of chondrocytes.

Research Project

Project/Area Number 06670049
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionTOKYO INSTITUTE OF TECHNOLOGY

Principal Investigator

HAGIWARA Hiromi  Tokyo Institute of Technology Faculty of Bioscience and Biotechnology Associate Professor, 生命理工学部, 助教授 (90189465)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordschondroclyte / natriuretic peptide / C-type natriuretic peptide / metabolism / proliferation / differentiation / cell physiology / エンドセリン / 受容体
Research Abstract

I investigated the effects of vasoactive peptides, natriuretic peptides (NP) and endothelin (ET) on cartilaginous metabolism. C-type natriuretic peptide (CNP) and B-type natriuretic peptide receptor (NPR-B) were present in cultured chondrocytes derived from rat xiphoid cartilage. Autocine CNP inhibited mitogenesis in chondrocytes via NPR-B under the control of TGF-beta and was involved in in vitro dedifferentiation. Furthermore, subtype switching of NPR during in vitro culture of rat chondrocytes was demonstrated by polymerase chain reaction analysis, receptor binding assay, and the cGMP formation method. Next, I examined the localization and characterization of endothelin receptor subtype in rat cartilages. Autoradiographic studies using ^<125>I-labeled endothelin-1 (ET-1) on sections of rat cartilage tissues, including the trachea, xiphisternum, and fetal rat epiphysis, revealed dense localization of endothelin receptors in the perichondrium. The perichondrial binding of ^<125>I-ET-1 was completely abolished wit BQ-123 (an endothelin receptor subtype A antagonist).Therefore, we demostrated the perichondrial localization of ETA receptors. [^3H] thymidine incorporation in vitro was significantly increased in rat xiphoid cartilage tissues exposed to ET-1.
These results suggest that NP system and ET system plays an important role in regulating cartilage metabolism and endochondral bone formation.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Hiromi Hagiwara: "Autocrine regulation of rat chondrocyte proliferation by natriuretic peptide C and its receptor,natriuretic peptide receptor-B." Journal of Biological Chemistry. 269. 10729-10733 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiromi Hagiwara: "Subtype switching of natriuretic peptide receptors in rat chondrocyte during in vitro culture." Journal of Biochemistry. 116. 606-609 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Khalid M.Lodhi: "Precondial localization of ET_A receptor in rat tracheal and xiphoid cartilage and infetal rat epiphysis." American Journal of Physiology. 268. C496-C502 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Atsuto Inoue: "Reciprocol regulation by cyclic nucleotides of the differentiation of rat osteoblast-like cells and mineralization of nodules." Biochem.Biophys.Res.Commun.215. 1104-1110 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiromi Hagiwara: "Changes in the expression of C-type natriuretic peptide and its receptor,B-type natriuretic peptide receptor during dedifferentiation of chondrocyte into fibroblast-like cells." Jourmal of Biochemistry. 119. 264-267 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 片渕 剛: "新型ナトリウム利尿ペプチド受容体(NPR-D)のクローニングと性質" 血圧. 2. 129-136 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 萩原啓実: "ナトリウム利尿ペプチドファミリー受容体---その発見から世界最初の臨床応用へ" 講談社, 204 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiromi Hagiwara, et.al.: "Autocrine regulation of rat chondrocyte proliferation by natriuretic peptide C and its receptor, natriuretic peptide receptor-B." J.Biological Chemistry. 269. 10729-10733 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiromi Hagiwara, et.al.: "Subtype switching of natriuretic peptide receptors in rat chondrocyte during in vitro culture." J.Biochemistry. 116. 606-609 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.M.Lodhi, et.al.: "Precondial localization of ET_A receptor in rat tracheal and xiphoid cartilage and infetal rat epiphysis." American Journal of Physiology. 268. C496-C502 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Atsuo Inoue, et.al.: "Reciprocol regulation by cyclic nucleotides of the differentiation of rat osteoblast-like cells and mineralization of nodules." Biochem.Biophys.Res.Commun.215. 1104-1110 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiromi Hagiwara, et.al.: "Changes in the expression of C-type natriuretic peptide and its receptor, B-type natriuretic peptide receptor during dedifferentiation of chondrocyte into fibroblast-like cells." J.Biochemistry. 119. 264-267 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.M.Lodhi, et al.: "Perichondrial localization of ETA receptor in rat tracheal and xiphoid cartilage and in fetal rat epiphysis" American Journal of Physiology. 268. C496-C502 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Atsuto Inoue, et al.: "eciprocol regulation by cyclic nucleotides of the differentiation of rat osteoblast-like cells and mineralization of nodules" Biochemical and Biophysical Research Communication. 215. 1104-1110 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hiromi Hagiwara, et al.: "Change in the expression of C-type natriuretic peptide and its receptor, B-type natriuretic peptide receptor during dedifferentiation of chondrocyte into fibroblast-like cells." Journal of Biochemistry. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 片渕 剛: "新型ナトリウム利尿ペプチド受容体(NPR-D)のクローニングと性質" 血圧. 2. 129-136 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 萩原啓実: "ナトリウム利尿ペプチドファミリー受容体---その発見から世界最初の臨床応用へ" 講談社, 204 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hiromi Hagiwara,et al: "Autocrine regulation of rat chondrocyte proliferation by natriuretic peptide C and its receptor,natriuretic peptide receptor-B" The Journal of Biological Chemistry. 269. 10729-10733 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hiromi Hagiwara,et al: "Subtype switching of ET natriuretic peptide receptors in rat chondrocytes during in vitro culture" Journal of Biochemistry. 116. 606-609 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.M.Lodhi,et al: "Perichondrial localization of receptor in rat tracheal and xiphoid cartilage and in fetal rat epiphysis" American Journal of Physiology. (in press).

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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