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Regulation of stimulus-secretion coupling in receptor-stimulated adrenal chromaffin cells

Research Project

Project/Area Number 06670050
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionNiigata University

Principal Investigator

WARASHINA Akira  Niigata University, School of Medicine, Associate professor, 医学部, 助教授 (50064580)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsCa^<2+> store / chromaffin cells / muscarine / bradykinin / histamine / catecholamine / thapsigargin / ryanodine / カテコルアミン分泌 / 「刺激-分泌」連関 / レセプター刺激 / 細胞内カルシウムストア / マンガン消光法 / サプシガルギン / リアノジン
Research Abstract

Changes in[Ca^<2+>]i and catecholamine release in rat adrenal chromaffin ells stimulated by muscarine, bradykinin and histamine were analyzed. The continuous receptor stimulation induced a biphasic increase, a prominent initial transient followed by a sustained phase, in either[Ca^<2+>]i or secretion. The initial component of[Ca^<2+>]i change contained a contribution of Ca^<2+> released from intracellular Ca^<2+> stores. This release of Ca^<2+> was completely inhibited by either thapsigargin or ryanodine, suggesting that both inositol trisphosphte-and caffeine-sensitive channels reside on Ca^<2+> stores in rat chromaffin cells. It was found that the inhibition of Ca^<2+> release did not appreciably affect secretory responses evoked by these agonists in a medium containing Ca^<2+> at normal concentrations. Thus, both the initial transient and sustained secretion depend exclusively on Ca^<2+> entry following receptor stimulation. In a medium containing Ca^<2+> at as low concentration as 16 uM,the initial component of bradykinin-evoked secretion was sustained at 59% of that in the normal medium, but was reduced to 23% after inhibition of Ca^<2+> release from stores with thapsigargin. This suggests that Ca^<2+> released from stores mayreinforce the Ca^<2+> entry which is attenuated so much in Ca^<2+>-deficient medium or in some other condition that the Ca^<2+> entry alone can not evoke a robust secretion.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Warashina,A.: "Properties of intracellular calcium stores and their role in receptor-mediated catecholamine secretion in rat adrenal chromaffin cells." Biological Signals. 4. 195-205 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujiwara,N.: "Characterization of low pH-induced catecholamine secretion in the rat adrenal medulla." Journal of Neurochemistry. 62. 1809-1815 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Warashina,A.: "[Ca2+]i changes arising in individual guinea-pig chromaffin cells in response to various receptor agonists and their relation to catecholamine secretion in perfused adrenal gland." Biomedical Research. 15. 271-280 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Busik,J: "Effect of SK&F 96365 on nicotinic or muscarinic agonist-induced and spontaneous Ca2+ dynamics in rat adrenal chromaffin cells." Biomedical Research. 15. 155-163 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Warashina, A.& Fujiwara, N.: "Properties of intracellular calcium stores and their role in receptor-mediated catecholamine secretion in rat adrenal chromaffin cells." Biological Signals. 4. 195-205 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujiwara, N., Warashina, A.& Shimoji, K.: "Characterization of low pH-induced catecholamine secretion in the rat adrenal medulla." Journal of Neurochemistry. 62. 1809-1815 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Warashina, A., Habara, Y., Yamaguchi, K.& Kanno: "[Ca^<2+>]i changes arising in individual guinea-pig chromaffin cells in response to various receptor agonists and their relation to catecholamine secretion in perfused adrenal gland." Biomedical Research. 15. 271-280 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Busik, J., Habara, Y., Warashina, A.& Kanno, T.: "Effect of SK&F 96365 on nicotinic or muscarinic agonist-induced and spontaneous Ca^<2+> dynamics in rat adrenal chromaffin cells." Biomedical Research. 15. 155-163 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Warashina,A.: "Properties of intracellular calcium stores and their role in receptormediated catecholamine secretion in rat adrenal chromaffin cells." Biological Signals. 4. 195-205 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fujiwara,N.: "Characterization of low pll‐induced catecholamine secretion in the rat adrenal medulla." Journal of Neurochemistry. 62. 1809-1815 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Warashina,A.: "[Ca2+]i changes arising in individual guinea‐pig chromaffin cells in response to various receptor agonists and their relation to catecholamine secretion in perfused adrenal gland." Biomedical Research. 15. 271-280 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Busik,J: "Effect of SK&F 96365 on nicotinic or muscarinic agonist‐induced and spontaneous Ca2+ dynamics in rat adrenal chromaffin cells." Biomedical Research. 15. 155-163 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 藤原 直士: "Characterzation of low pll-induced catecholamine secretion in the rat adrenal medulla." Journal of Neurochemistry. 62. 1809-1815 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Julia Busik: "Effect of SK&F96365 on nicotinic or muscarinic agonist-induced and spontaneous Ca^<2+> dynamics in rat adrenal chromaffin cells." Biomedical Research. 15. 155-163 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 藁科,彬: "[Ca^<2+>]_1 changes arising in individual guinea-pig chromaffin cells in response to various receptor agonists and their relation to catecholamine secretion in the perfused adrenal galnd." Biomedical Research. 15. 217-280 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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