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Analyzes of the role of urokinase receptor cell growth

Research Project

Project/Area Number 06670052
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionHAMAMATSU UNIVERSITY SCHOOL OF MEDICINE

Principal Investigator

URANO Tetsumei  Hamamatsu Univ.Sch.of Med.Dept.of Physiology associate professor, 医学部, 助教授 (50193967)

Co-Investigator(Kenkyū-buntansha) NAGAI Nobuo  Hamamatsu Univ.Sch.of Med.Dept.of Physiology research associate, 医学部, 助手 (90260281)
TAKADA Yumiko  Hamamatsu Univ.Sch.of Med.Dept.of Physiology research associate, 医学部, 助手 (90092981)
TAKADA Akikazu  Hamamatsu Univ.Sch.of Med.Dept.of Physiology professor, 医学部, 教授 (80092980)
井原 勇人  浜松医科大学, 医学部, 助手 (00223298)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsurokinase / urokinase receptor / plasminogen activator / plasminogen activator inhibitor / cell growth / プラスミノーゲン・アクチベータ- / プラスミノーゲン・アクチベータ-・インヒビター / プラスミノーゲン アクチベータ- / プラスミノーゲン アクチベータ- インヒビター
Research Abstract

(1) We studied the expression of uPAR mPNA in cultured cell line (U-937). The expression of uPAR mRNA was enhanced by phorbol-ester stimulation, which resulted in the higher accumulation of uPAR antigen both in the medium and in the cell lysate. We detected two different forms of uPAR mRNA,one of which coodes mature uPAR and another codes soluble uPAR.Employing two different DNA probes, each of which detects only one of these two different mRNAs, we demonstrated that both forms increased after phorbol ester stimulation.
(2) We assayd antigen levels of soluble uPAR in cancer patients plasma. In lung cancer patients, plasma soluble uPAR levels were significantly higher than those in normal controls. Either by proteolytic cleavage of mature uPAR or by increasing alternatively spliced mRNA for soluble uPAR,plasma uPAR seems to be elevated. Plasma soluble uPAR level might be a useful tumor marker of lung cancer.
(3) The expression of uPA mRNA in lung cancer tissue was significantly higher than that in normal lung tissue. The expression of uPAR mRNA,however, did not differ between cancer tissue and normal lung tissue. mRNA of both PAI-1 and PAI-2 were also higher in lung cancer tissue than those in normal counter parts, which are in accordance with our previous results of their antigen level.
(4) We analyzed the effects of either uPA alone or of uPA together with its specific inhibitor on cell growth using U937. uPA alone slightly enhanced cell growth. Simultancous use of its inhibitor, however, did not influence cell growth. Since U937 without treatment by phorbol-ester may not have enough amounts of uPAR on cell surface, further experiment using other cell line must be needed to clarify the effect of uPA and its inhibitors on cell growth.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (54 results)

All Other

All Publications (54 results)

  • [Publications] Nagayama,M.et al: "Plasminogen activators and their inhibitors in non-small cell lung cancer:Low content of type 2 plasminogen activator inhibitor associated with tumor dissemination." Cancer. 73(5). 1398-1405 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takada,Y.et al: "Effect of heparan sulfate analogue or other sulfated polysaccharides on the activation of plasminogen by t-PA or u-PA." Thromb.Res. 73(5). 301-311 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Urano T.et al: "Electric-foot-shock induced the supression of fibrinolytic activity in rats." Thromb.Res.74(6). 595-603 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kojima,Y.et al: "The Significant Enhancement of Fibrinolysis by Calcium Ion in a Cell Free System.:The Shortening of Euglobulin Clot Lysis Time by Calcium Ion." Thromb.Haemostas. 72(1). 113-118 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Urano,T.et al: "Heparin and heparan sulphate enhancement of the inhibitory activity of plasminogen activator inhibitor type 1 toward urokinase type plasminogen activator." Biochim.Byophys.Acta. 1201. 217-222 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Serizawa,K.et al: "Medroxyprogesterone acetate (mpa) increases PAI-1 secretion from huvec and elevates the plasma levels of PAI-1 in vivo." Oneology Report. 1. 1127-1130 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Malyozko,J.et al: "Correlation between platelet aggregation,fibrinolysis,peripheral and central serotonergic measures in subhuman primates." Afherosclerosi. 110. 63-68 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Malyozko,J.et al: "Relationships between serum lipids,serotonin,platelet aggregation and some fibrinolytic parameters in humans." Life Sciences. 55(21). 1619-1623 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takada,A.et al: "The physiological aspects of fibrinolysis.(Review)" Thromb.Res.76(1). 1-31 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 浦野哲盟 等: "網膜静脈血栓症に対する新しい線溶療法:糖尿病と血栓" 眼科紀要. 45. 1125-1130 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 浦野哲盟: "t-PAの分子構造と血栓溶解機構" 病態生理. 13(9). 673-679 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kotani,I.,et al: "Increased procoagulant and antifibrinolytic activities in the lungs with idiopathic pulmonary fibrosis." Thromb.Res.77(6). 493-504 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Wu,K.,et al: "The Cleavage and Inactivation of Plasminogen Activator Inhibitor Type 1 by Neutrophil Elastase: The Evaluation of Its Physiological Relevance in Fibrinolysis." Blood. 86. 1056-1061 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Urano,T.,et al: "Close relationships between serotonergic and fibrinolytic systems revealed by a mono-amine-oxidase inhibitor treatment in rats." Haemostasis. 25(6). 277-282 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Urano,T.,et al: "Absence of synergism in the activity of tissue plasminogen activator and urinary plasminogen activator measured by clot lysis time." Turk J Haematol.14. 3-7 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Urano,T.,et al: "Reexamination of the extent of the activation of lys78-plasminogen by tissue plasminogen activator in the presence of polymerized fibrin." Haemostasis. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 浦野哲盟 等: "ストレス負荷による血小板機能及び線溶活性の変化と、血中セロトニン関連物質との関係" 生理学研究所年報. 16. 334- (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 浦野哲盟 等: "plasminogen activator inhibitor type 1 (PAI-1)による線溶活性制御機構" 血液と循環. 9(2). 7-14 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nagayama, M.et al: "Plasminogen activators and their inhibitors in non-small cell lung cancer. Low content of type 2 plasminogen activator inhibitor associated with tumor dissemination." Cancer.73 (5). 1398-1405 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takada, Y.et al: "Effect of heparan sulfate analogue or other sulfated polysaccharides on the activation of plasminogen by t-PA or u-PA." Thromb.Res.73 (5). 301-311 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Urano T.et al: "Electric-foot-shock induced the supression of fibrinolytic activity in rats." Thromb.Res.74 (6). 595-603 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kojima, Y.et al: "The Significant Enhancement of Fibrinolysis by Calcium Ion in a Cell Free System. : The Shortening of Euglobulin Clot Lysis Time by Calcium Ion." Thromb. Haemostas.72 (1). 113-118 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Urano, T., et al: "Heparin and heparan sulphate enhancement of the inhibitory activity of plasminogen activator inhibitor type 1 toward urokinase type plasminogen activator." Biochim. Byophys. Acta.1201. 217-222 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Serizawa, K., et al: "Medroxyprogesterone acetatc (mpa) increases PAI-1 secretion from huvec and elevates the plasma levels of PAI-1 in vivo." Oncology Report. 1. 1127-1130 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Malyszko, J., et al: "Correlation between platelet aggregation, fibrinolysis, peripheral and central serotonergic measures in subhuman primates." Atherosclerosi. 110. 63-68 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Malyszko, J., et al: "Relationships between serum lipids, serotonin, platelet aggregation and some fibrinolytic parameters in humans." Life Sciences. 55 (21). 1619-1623 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takada, A., et al: "The physiological aspects of fibrinolysis. (Review)" Thromb.Res.76 (1). 1-31 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kotani, I., et al: "Increased procoagulant and antifibrinolytic activities in the lungs with idiopathic pulmonary fibrosis." Thromb.Res.77 (6). 493-504 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Wu, K., et al: "The Cleavage and Inactivation of Plasminogen Activator Inhibitor Type 1 by Neutrophil Elastase : The Evaluation of Its Physiological Relevance in Fibrinolysis." Blood. 86. 1056-1061 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Urano, T., et al: "A.Close relationships between serotonergic and fibrinolytic systems revcaled by a mono-amine-oxidase inhibitor treatment in rats." Haemostasis. 25 (6). 277-282 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Urano, T., et al: "Absence of synergism in the activity of tissue plasminogen activator and urinary plasminogen activator measured by clot lysis time." Turk J Haematol.14. 3-7 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Urano, T., et al: "Reexamination of the extent of the activation of lys78-plasminogen by tissue plasminogen activator in the presence of polymerized fibrin." Haemostasis. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nagayama,M.et al: "Plasminogen activators and their inhibitors in non-small cell lung cancer.Low content of type 2 plasminogen activator inhibitor associated with tumor dissemination." Cancer. 73(5). 1398-1405 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takada,Y.et al: "Effect of heparan sulfate analogue or other sulfated polysaccharides on the activation of plasminogen by t-PA or u-PA." Thromb. Res.73(5). 301-311 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Urano T.et al: "Electric-foot-shock induced the supression of fibrinolytic activity in rats." Thromb. Res.74(6). 595-603 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kojima,Y.et al.: "The Significant Enhancement of Fibrinolysis by Calcium Ion in a Cell Free System. :The Shortening of Euglobulin Clot Lysis Time by Calcium Ion." Thromb. Haemostas.72(1). 113-118 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Urano,T.,et al.: "Heparin and heparan sulphate enhancement of the inhibitory activity of plasminogen activator inhibitor type 1 toward urokinase type plasminogen activator." Biochim, Byophys. Acta. 1201. 217-222 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Serizawa,K.,et al.: "Medroxyprogesterone acetate(mpa)increases PAI-1 secretion from huvec and elevates the plasma levels of PAI-1 in vivo." Oncology Report. 1. 1127-1130 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Wu,K.,et al: "The Cleavage and Inactivation of Plasminogen Activator Inhibitor Type 1 by Neutrophil Elastase: The Evaluation of Its Physiological Relevance in Fibrinolysis." Blood. 86. 1056-1061 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Urano,T.,et al: "A. Close relationships between serotonergic and fibrinolytic systems revealed by a mono-amine-oxidase inhibitor treatment in rats." Haemostasis. 25(6). 277-282 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Urano,T.,et al: "Absence of synergism in the activity of tissue plasminogen activator and urinary plasminogen activator measured by clot lysis time." Turk J Haematol.14. 3-7 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Urano,T.,et al: "Reexamination of the extent of the activation of lys 78-plasminogen by tissue plasminogen activator in the presence of polymerized fibrin." Haemostasis.

    • Related Report
      1995 Annual Research Report
  • [Publications] 浦野哲盟等: "ストレス負荷による血小板機能及び線溶活性の変化と、血中セロトニン関連物質との関係" 生理学研究所年報. 16. 334- (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 浦野哲盟等: "plasminogen activator inhibitor type 1(PAI-1)による線溶活性制御機構" 血液と循環. 9(2). 7-14 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Malyszko,J.,et al: "Correlation between platelet aggregation, fibrinolysis, peripheral and central serotonergic measures in subhuman primates." A therosclerosi. 110. 63-68 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Malyszko,J.,et al: "Relationships between scrum lipids, serofonin, platelet aggregation and some fibrinolytic parameters in humans." Life Sciences. 55(21). 1619-1623 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takada,A.,et al: "The physiological aspects of fibrinolysis. (Review)" Thromb. Res.76(1). 1-31 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 浦野哲盟等: "網膜静脈血栓症に対する新しい線溶療法:糖尿病と血栓" 眼科紀要. 45. 1125-1130 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 浦野哲盟等: "t-PAの分子構造と血栓溶解機構" 病態生理. 13(9). 673-679 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kotani,I.,et al: "Increased procoagulant and antifibrinolytic activities in the lungs with idiopathic pulmonary fibrosis." Thromb. Res.77(6). 493-504 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Izumi Kotani et al: "Increased procoagulant and antifibrinolytic activities in the lungs with idicpathic pulmonary fibrosis" Thrombosis Research. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Kiyohito Serizawa et al: "Medroxyprogesterone acetate (MPA) iwreases PAI-1 secretion from HUVEC and elevates plasma livels of PA-in" Oncology Reports. 1. 1127-1130 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tetsumei Urano ey al: "Heparin and heparam sutfate enhanernent of the inhibitory activity of plasminogen activator inhibitor type1 toward urokinase type plasminogen activator" Biochimica et Biophysica Acta. 1201. 217-222 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 浦野哲盟 他: "網膜静脈閉塞症に対する線溶療法:糖尿病と血栓" 眼科紀要. 45. 1125-1130 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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