| Project/Area Number |
06670121
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General pharmacology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
MIYAMOTO Atsushi Sapporo Medical University, School of Medicine, Department of Pharmacology, Associate Professor., 医学部, 助教授 (50166196)
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| Co-Investigator(Kenkyū-buntansha) |
OYAMADA Masahito Sapporo Medical University, school of Medicine, Department of Pathology, Assista, 医学部, 講師 (30183255)
KIMURA Hisakazu Sapporo Medical University, School of Medicine, Department of Pharmacology, Inst, 医学部, 助手 (20204985)
OHSHIKA Hideyo Sapporo Medical University, School of Medicine, Department of Pharmacology, Prof, 医学部, 教授 (50045358)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Intercellular channel proteins / Gap-junction / Synchronized contraction / Connexin 43 / Cardiac myocytes / Excitaion-contraction coupling / フォトニックセンサー / 培養心筋細胞 / 加齢 / 自動拍動 |
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| Research Abstract |
We analyzed the expression, phosphorylation, and localization of the major cardic gap-junction protein connexin 43 during the establishment of a synchronized contraction in confluent monolayrs of primary cultured neonatal rat cardiac myocytes, combined with a functional assay of gap junctions by the microinjection-dye transfer method. The findings suggested that a concerted action of the expression, phosphorylation, and localization of connexin 43 and gap-junctional intercellular communication plays a major role in the reestablishment of synchronous beating of cultured neonatal rat cardiac myocyte. (Exp. Cell Res., 212,351-358,1994)
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