| Project/Area Number |
06670125
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General pharmacology
|
|---|
| Research Institution | Tokyo Women's Medical College |
|---|
Principal Investigator |
TSUKAHARA Fujiko Tokyo Women's Medical College, Medical dept., Instructor, 医学部, 助手 (40119996)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MURAKI Takamura Tokyo Women's Medical College, Medical dept., Professor, 医学部, 教授 (50051446)
IRIE Kaoru Tokyo Women's Medical College, Medical dept., Instructor, 医学部, 助手 (50075496)
|
|---|
| Project Period (FY) |
1994 – 1995
|
| Project Status |
Completed (Fiscal Year 1995)
|
| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥1,600,000 (Direct Cost: ¥1,600,000)
|
|---|
| Keywords | thyroid hormone / thyroxine / deiodinase / cDNA / human / rat / PCR / cloning / サイロキシン5'-脱ヨード酵素 / 甲状腺ホルモン代謝 / トリヨードサイロニン / 脳 / 遺伝子 |
|---|
| Research Abstract |
We cloned and analyzed thyroid hormone type II 5'-deiodinase (5'DII) and type III 5-deiodinase (5DIII) cDNA.1) Cloning of 5'DII cDNA.Based on sequence homology between human and rat type I 5'-deiodinase, we prepared several primers for 5'DII and 5DIII.RNA was isolated from rat brain, pituitary and brown adipose tissue and subjected to PT-PCR.Among several PCR products, three DNA fragments, the abundance of which were increased when 5'DII activity was induced by thyroidectomy or cold exposure, were subcloned and sequenced. Database search revealed that these DNA fragments showed no homology to known genes, suggesting the possibility that they may be some parts of rat brain 5'DII cDNA.Detailed analysis of these DNA fragments is in progress. 2) Cloning of 5DIII cDNA.We analyzed human brain and placenta cDNA libraries by PCR using several primers described above. A PCR product which assumed to be 5DIII was subcloned and sequenced. Homology arch revealed that this DNA fragment is homologous to a part of rat 5DIII cDNA.Thus, we certainly could isolate a part of human 5DIII.
|
