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Structure and Function of Novel Cytokine, SDF-1

Research Project

Project/Area Number 06670139
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionOsaka University (1995)
Kyoto University (1994)

Principal Investigator

NAKANO Toru  Osaka University, Research Institute for Microbial Diseases, Department of Molecular Cell Biology, Professor, 微生物病研究所, 教授 (00172370)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
KeywordsCytokine / Chemokine / Chromosome mapping / インタークライン / 遺伝子構造
Research Abstract

Stromal cell-derived factors 1alpha and 1beta are small cytokines belonging to the intercrine CXC subfamily and originally isolated from a murine bone-marrow stroma cell line by the signal seqluence trap method. cDNA and genomic clones of human SDF1alpha and SDF1beta (SDF1A and SDF1B) were isolated and characterized. cDNAs of SDF1alpha and SDF1beta encode proteins of 89 and 93 amino acids, respectively. SDF1alpha and SDF1beta sequences are more than 92% identical to those of the human conterparts. The genomic structure of the SDF1 gene revealed that human SDF1alpha and SDF1beta are encoded by a single gene and arise by altemative splicing. SDF1alpha and SDF1beta are encoded by 3 and 4 exons, respectively. Ubiquitous expression of the SDF1 gene, except in blood cells, was consistent with the presence of the GC-rich sequence in the 5' flanking region of the SDF1 gene, as is often the case in the "house keeping" genes. Although genes encoding other members of the intercrine family are localized on chromosome 4q or 17q, fluorescence in situ hybridization. Strong evolutionary conservation and unique chromosomal lacalization of the SDF1 gene suggest that SDF1alpha and SDF1beta may have important functions distinct from those of other members of the intercrine family.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] M. Shirozu, T. Nakano, J. Inazawa, K. Tashiro, H. Tada, T. Shinohara, T. Honjo: "Structure and Chromosomal Localization of the Human Stromal Cell-Derived Factor 1 (SDF1) Gene" Genomics. 28. 495-500 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Shirozu, T.Nakano, J.Inazawa, K.Tashiro, H.Tada, T.Shinohara, T.Honjo: "Structure and chromosomal localization of the human stromal cell-derived factor 1 (SDF-1) gene" Genomics. vol.28. 495-500 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shirozu M et al.: "Structure and chromosomal localization of the human stromal cell-derived factor 1(SDF-1)gene" Genomics. 28. 495-500 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Nakano,H.Kodama T.Honjo: "Generation of lymphohematopoietic cells from embryonic stem cells in culture" Science. 265. 1098-1101 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Nakano: "Lymphohematopoietic development of embryonic stem cells in vitro" Seminars in Immunology. (in press). (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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