| Project/Area Number |
06670175
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Kurume University School of Medicine |
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Principal Investigator |
YAMADA Akira Kurume Univ.Sch.Med., Dept.Immunol., Assistant Professor, 医学部, 講師 (50158177)
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| Co-Investigator(Kenkyū-buntansha) |
SHICHIJO Shigeki Kurume Univ.Sch.Med., Dept.Immunol., Associate Professor, 医学部, 助教授 (30080592)
HOSHINO Tomoaki Kurume Univ.Sch.Med., Dept.Immunol., Instructor, 医学部, 助手 (00261066)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | LFA-1-like molecule / LFA-1 / CD11a / Adhesion molecule / Exocrine glands / Malignant tumors / adenocarcinoma |
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| Research Abstract |
We previously reported that the immunohistochemically defined LFA-1 antigen (LFA-1-like antigen) was expressed on various exocrine tissues uninvolved by tumors in patients with malignant diseases using LFA-1alpha-specific monoclonal antibodies (mAb) 2F12 and HVS6B6. We investigated in this manuscript differences at the molecular level between LFA-1 on leukocytes and LFA-1-like antigen on MKN-45.16, a subline derived from an adenocarcinoma line MKN45 that expresses a high amount of LFA-1-like antigen. LFA-1-like antigen was reactive to mAb 2F12 or HVS6B6, but not to any of the other five different LFA-1alpha (CD11a) -specific or four LFA-1beta (CD18) -specific mAb. MAb 2F12 immunoprecipitated a 200 KD molecular weight membrane protein (LFA-1-like antigen) from MKN45.16 cells, whereas it precipitated 180 KD (LFA-1alpha) and 95 KD (LFA-1beta) proteins form a monocytic cell line (THP-1) under both non-reducing and reducing conditions. The molecular difference was ocnfirmed further by N-glycanse treatment of the immunoprecipitates. The isoelectric point of LFA-1-like antigen was 6.0, whereas that of LEA-1alpha and LFA-1beta was 6.0 and 4.7, respectively, by two-dimensional gel electrophoresis. Expression of LFA-1alpha gene on MKN45.16 cells was not detected at the mRNA level by six different sets of LFA-1alpha specific oligonucleotides and reverse transcription-polymerase chain reaction. These results indicated that LFA-1-like antigen on an adenocarcinoma cell line was a distinct molecule from LFA-1 on leukocyte.
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