• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

xpression, regulation and poly morphism of the uxil geuls

Research Project

Project/Area Number 06670214
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionChiba university

Principal Investigator

SIMIZU Bunshiti  Chiba University School of Medicine, Professor, 医学部, 教授 (10072894)

Co-Investigator(Kenkyū-buntansha) SHIRASAWA Hiroshi  Chiba University School of Medicine, Assistant, 医学部, 助手 (00216194)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsnearoblastoma cells / differentiation / mxil / Mxi1
Research Abstract

The myc proto-oncogene family plays an important role in the control of cellular growth and fifferentiation. Mxil, one of the Max-associated proteins, has been known to have an antagonistic action on Myc activity.
We have cloned and sequenced the murine Mxil gene encoding Mxil, one of Max-associated proteins. Murine and human sequences showed 87.9% nucleotide (nt) and 90.3% Amino acid (aa) sequence homology, whereas murine and zebra fish sequences showed 67.2% nt and 67.8% aa sequence homology.
Using this Mxil cDNA,we analyzed the expression of the gene during differentiation of neuroblastoma cells. The mxil mRNA increased during growth inhibition and differentiation, and decreased with serum stimulation in mammal cell lines. We have also found an AAAAC polymorphic repeat in the 3' non-coding region of the human mxil cDNAs and a difference between the mxil mRNA half-lives in some different cell lines.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Shimizu E.et al.: "Cloning and sequencing of the murine Mxil cDNA" Gene. 152. 283-284 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shimizu E.et al.: "Expression, requlation and polymorphism of the mxil genes" Gene. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsunaga et al.: "Expression of neuronal src mRNA as a favorable marker and inverse conelation to N-mye gene amplification in human neusoblastomas" International Journal of Cancer. 58. 793-798 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shimizu, E., Shirasawa, H., Kodama, K., Koseki, K., Sato, T., and Simizu, B.: "Cloning and sequencing of the murine Mxil cDNA." Gene. 152. 283-284 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shimizu, E., Shirasawa, H., Kodama, K., Sato, T., and B.Simizu.: "Expression, regulation and polymorphism of the mxil genes." Gene. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shimizu,E.et al.: "Cloning and sequencing of the murine Mxil cDNA" Gene. 152. 283-284 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shimizu,E.et al.: "Expression,regulation and poly morphism of the mxil genes" Gene. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Matsunaga,T.et.al.: "Expression of neuronal src mRNA as a favorable marker and inverse correlation to H myc gene amplification in human newoblastomas" International Journal of Cancer. 58. 793-798 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] E.Shimizu.et.al.: "cloning and sequencing of the murine Mxi1 cDNA" Gene. 152. 283-284 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Shirasawa et al.: "Transcription-modulatory activitiy of full-length E6 and E6^*L proteins of human papillomavirus type 16" Virology. 203. 36-42 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Matsunaga et al.: "Expression of neuronal src mRNA as a favorable marker and inverse correlation to N-myc gene amplification in human neuroblas tomas" Int.J.Cancer. 58. 793-798 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Shimizu et al.: "Alternative splicing of human papillomavirus type-16 E6 mRNA in mouse and monkey cell lines" Int.J.Oncology. 4. 971-976 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] N.Isegawa et.al.: "Cloning and sequencing of the L1 gene of canine oral papillomavirus" Gene. 146. 261-265 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] N.Akutsu et al.: "Rare association of human papillomavirus DNA with esophageal cancer in Japan." J.infect.Disease. 171. 425-428 (1995)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi