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Regulation loss of mesangial cell function leading to irreversible, glomerular sclerosis.

Research Project

Project/Area Number 06670215
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionNiigata University

Principal Investigator

OITE Takashi  Niigata Univ. Sch. Medicine Associate Professor, 医学部, 助教授 (60018744)

Co-Investigator(Kenkyū-buntansha) MORIKA Tetsuo  Niigata Univ. Sch. Medicine Assistant Professor, 医学部, 助手 (00210146)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsrenal glomerulus / mesangial cell / vascular endothelial cell / extracellular matrix / cell adhesion molecules / cell-cell interaction / regulation of cell function
Research Abstract

In the glomerulus, the endocapillary region is a closed space surrouded by the glomerular basement membrane comprised of a capillary lumen, endothelial cells (ECs), mesangial cells (MCs), and mesangial matrices. Our working hypothesis is that loss of morphological and functional regulation by intrinsic cells and/or matrices may induce MCs dysfunction, leading to the pathological changes such as proliferation, enhanced extracellular matrix synthesis, MCs migration and mesangial interposition in the endocapillary region. By the aid of research grant (No.06670215,1994) from the Ministry of Education, Science and Culture, we were able to establish the culture method of rat aortic ECs (T Oite el al. MicrovascRes50 : 113,1995). Using these ECs, we have examined the potentially functional moleculepresent on rat MCs surface in coculture system. During the research period (1994-1995) supported by the above described grant, we have obtained the following results ; 1.Some specific molecules, associated with Thy-1.1 antigen was preseent on cultured rat MCs. One ofthem had a specific epitope which was concentrated on the side of the MCs that faces with ECs in coculture system .2.COS cells transfected with cDNA for Thy-1.1 ware able to be used for analysis of the relevant epitope (T Oite etal. Exp Nephrol 1996, in press) . 3.An animal model of progressive glomerulosclerosis was developed by administeringa single iv injection of monoclonal antibody against this epitope to unilaterally nephrectomized rats (Cheng QL et al Clin exp Immunol 102 : 181,1995). 4.Expression of collagen type I and III was increased in glomeruli from these rats at the levels of protein and mRNA.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] H.Kawachi et.al.: "Effct of traditional chinese medicine …… in rats." Pathol Int. 44. 339-344 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Joh et.al.: "Selective planting of cationized …… on the rat." Virchows Arch. 424. 587-591 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Kuroda et.al.: "Nephrotoxic serum nephritis …… cell-mediated immunity." Nephron. 68. 360-365 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 森岡哲夫,追手巍: "In situ IC 形成." 腎と透析(臨時増刊号). 37. 154-158 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Tomita et.al: "Possible significance of VLA-4 …… renal cell cancer." Int J Cancer. 60. 753-758 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] D.Gollner et.al.: "Strain variation in susceptibility …… in rats." Clin exp Immunol. 102. 181-185 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 追手 巍: "糸球体細胞機能-内皮細胞" 腎と透析(増刊号・腎と高血圧). 39. 29-34 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Morioka et.al.: "Anti-DNA antibody derived …… glomeruli in vivo." Clin exp Immunol. (印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Oite.,Y.Suzuki.,T.Morioka.,M.Saito.,F.Shimizu: "Efficient isolation of rat aortic endothelial cells by elimination of contaminating cells with a monoclonal antibody." Microvase.Res.50. 113-118 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Oite.,M.Saito.,Y.Suzuki.,T.Arii.,T.Morioka.,F.Shimizu: "A specific Thy-a molecular epitope expressed on rat mesangial cells." Exp.Nephrol.印刷中 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 追手 巍: "免疫実験操作法(分担:腎炎)" 右田,紺田,本庶,濱岡 編,南江常, 1236(1181-1184) (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 追手 巍: "金沢腎セミナー講演集(半月体の細胞組成)" 木田寛 編, 40(8-14) (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Oite.,M.Saito.,T.Morioka: "Recent Advances in Molecular Nephrology (Modulation of mesangial cell …… and matrices)" edited by M.Arakawa,Y.Nakagawa (Kohko-Do,Niigata,Japan), 119(98-112) (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H Kawachi et al: "Effect of traditional chinese medicine・・・・・・" Pathol Int. 44. 339-344 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K Joh et al: "Selective planting of cationized・・・・・・" Virchows Arch. 424. 587-591 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Kuroda et al.: "Nephrotoxic serum nephritis・・・・・・" Nephron. 68. 360-365 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Tomita et al.: "Possible significance of VLA-4・・・・・・" Int J Cancer. 60. 753-758 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Oite et al.: "Efficient isolation of rat aortic・・・・・・" Microvasc Res. 50. 113-118 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] D.Gollner et al.: "Strain variation in susceptibility・・・・・・" Clin exp Immunol. 102. 181-185 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Morioka et al.: "Anti-DNA antibody derived・・・・・・" Clin exp Immunol. in press. (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Oite et al.: "A specific Thy-1 molecular・・・・・・" Exp Nephrology. in press. (1966)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Kawachi et.al.: "Effect of traditional chinese medicine ……in rats." Pathol Int. 44. 339-344 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Joh et.al.: "Selective planting of cationiged……on the rat." Virchows Arch. 424. 587-591 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Kuroda et.al.: "Nephrotoxic serum nephritis……cell-mediated immunity." Nephron. 68. 360-365 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 森岡哲夫.追手巍: "In situ IC形成" 腎と透析. 37(臨時増刊号). 154-158 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Tomita et.al.: "Possible significance of VLA-4……renal cell cancer." Int J Cancer. 60. 753-758 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Oite et.al.: "Efficient isolation of rat aortic……antibody." Microvasc Res. 50. 113-118 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] D.Gollner et.al.: "Strain variation in susceptibility……in rats." Clin exp Immunol. 102. 181-185 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 追手巍: "糸球体細胞機能-内皮細胞" 腎と透析(増刊号.腎と高血圧). 39. 29-34 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Morioka et.al.: "Anti-DNA antibody derived……glomeruli in vivo." clin exp Immunol. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Oite et.al.: "A specific Thy-1 molecular epitope……mesangial cells." Exp Nephrol. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 追手 巍: "免疫実験操作法(分担・腎炎)" 右田,紺田,本庶,濱岡編,南江常, 1236(1181-1184) (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 追手 巍: "金沢腎セミナー講演集(半月体の細胞組成)" 木田寛編, 40(8-14) (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Oite et.al: "Recent Advances in Molecular Nephrology(Modulation……)" ed. M.Arakawa, Y.Nakagawa,Kohoko-Do, 119(98-112) (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Oite et.al.: "Eficient isolation of rat aorticendothelial cells.....antibody." Microvascular Research. 印刷中. (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 追手 巍: "免疫実験操作法,腎炎" 南江堂(右田,紺田,本庶,濱岡編), 4 (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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