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Cloning of genes expressing in the early stage of rat bladder carcinogenesis induced by BBN

Research Project

Project/Area Number 06670224
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

ONO Takahiko (1995)  Kyoto Univ., Graduate School of Medicine Research Associate, 医学研究科, 助手 (60243028)

杉山 武敏 (1994)  京都大学, 医学部, 教授 (20030851)

Co-Investigator(Kenkyū-buntansha) SHIMADA Toshihide  Kyoto Univ., Graduate School of Medicine Research Associate, 医学研究科, 助手 (30231690)
TAKAHASHI Rei  Kyoto Univ., Graduate School of Medicine Associate Professor, 医学研究科, 助教授 (60144565)
逢坂 光彦  京都大学, 医学部, 助手 (20252463)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsUrethelial cancer / Tumor suppressor / Carcinogenesis / Cloning / PCR / Chemical carcinogenesis / ラット発癌 / BBN / 遺伝子クローニング / LOH
Research Abstract

We have been studying inactivation of tumor suppressor genes in human urothelial cancer cells. Along with analyzes using human tumor tissues, experimental models should provide data in the early stage of carcinogenesis or in precancerous lesions. A recently developed technique of differential RT-PCR allows us to detect expression of genes highly expressed in such primary lesions. It would also be possible to identify human homologues from clones isolated by the differential RT-PCR method. Total cytoplasmic RNA was isolated from hyperplastic lesions, papilloma, and carcinoma in situ of the rat bladder treated with BBN for six months. Differential display was performed with several sets of random primers and oligo dTs using a normal bladder mucosa as a control. Forty-two bands were chosen because they were specificly found or their intensity was increased. Confirmation of specific expression in each lesions was performed using Northern blot or quantitative RT-PCR assay. Due to a limited amount of RNA from the primary lesions, quantitative RT-PCR was effective for such evaluation. Further characterization of the isolated clones are ongoing.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Ito Y.: "Long-evans A and C rat strains susceptible to clastogenic effects of chemicals in the bone marrow cells" Jpn. J. Cancer Res.85. 26-31 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kinoshita H.: "Contribution of chromosome 9p21-22 deletion to the progression of human renal cell carcinoma" Jpn. J. Cancer Res.86. 795-799 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kho T.: "Alternative splicing of the neurofibromatosis 1 gene correlates with growth patterns and neuroendocrine properites of humman small cell lung carcinoma cells" Int. J. Cancer. 60. 843-847 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ping Liang: "Chromosomal localization of the rat erythropoietin receptor gene by fluorescence in situ hybridzation" Jpn. J. Genet.70. 525-531 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tamura T.: "Induction of Fas-mediated apoptosis in p53-transfected human colon carcinoma cells" Oncogene. 11. 1939-1946 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Suzuki M.: "Overexpression of p53, c-erbB-2 and epidermal growth factor receptor in human breast carcinoma" Pathology International. 46. 46-53 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ito Yoshiaki: "Long-Evans A and C rat strains susceptible to clastogenic effects of chemicals in the bone marrow cells" Jpn.J.Cancer Res.85. 26-31 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kinoshita Hidefumi: "Contrubution of chromosome 9p21-22 deletion of the progression of human renal cell carcinoma" Jpn.J.Cancer Res.86. 795-799 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kho Takashi: "Alternative splicing of the neurofibromatosis 1 gene correlates with growth patterns and neuroendocrine properites of human small cell lung carcinoma cells" Int.J.Cancer. 60. 843-847 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ping Liang: "Chromosomal localization of the rat erythro-poiethin receptor gene by fluorescence in situ hybridiztion" Jpn.J.Genet.70. 525-531 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tamura Takao: "Induction of Fas-mediated apoptosis in p53 transfected human colon carcinoma cells" Oncogene. 11. 1939-1946 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Suzuki Masatsugu: "Overexpression of p53, c-erbB-2 and epidermal growth factor receptor in human breast carcinoma" Pathology Int.46. 46-53 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ito Y.: "Long-Evans A and C Rat Strains Susceptible to Clastogenic Effects of Chemicals in the Bone Marrow Cells" Jpn. J. Cancer Res.85. 26-31 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kinoshita H.: "Contribution of Chromosome 9p21-22 Deletion to the Progression of Human Renal Cell Carcinoma" Jpn. J. Cancer Res.86. 795-799 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kho T.: "Alternative splicing of the neurofibromatosis 1 gene correlates with growth patterns and neuroendocrine properites of human small cell lung carcinoma cells" Int. J. Cancer. 60. 843-847 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ping Liang: "Chromosomal localization of the rat erythropoietin receptor gene by fluorescence in situ hybridization" Jpn. J. Genet.70. 525-531 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tamura T.: "Induction of Fas-mediated apoptosis in p53-transfected human colon carcinoma cells" Oncogene. 11. 1939-1946 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Suzuki M.: "Overexpression of p53, c-erbB-2 and epidermal growth factor receptor in human breast carcinoma" Pathology International. 46. 46-53 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ito Yoshiaki: "Long-Evans A and C Rat Strains Susceptible to Clastogenic Effects of Chemicals in the Bone Marrow Cells" Japan Journal of Cancer. 85. 26-31 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Osaka Mitsuhiko: "N-ras mutation in 7,12-dimethylbenz(a) anthracene(DMBA)-induced erythroleukemia in Long-Evancs rats" Cancer Letters. (印刷中).

    • Related Report
      1994 Annual Research Report
  • [Publications] Liang Ping: "Chromosomal localization of rat erythropoietin receptor gene by fluorescence in situ hybridization" Cytogenetics and Cell Genetics. (印刷中).

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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