Elucidation of the proliferative mechanism of fat cells : Abasic study of the obesity
| Project/Area Number |
06670230
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Saga Medical School |
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Principal Investigator |
SUGIHARA Hajime Saga Medical School, Faculty of Medicine, Professor, 医学部, 教授 (50039509)
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| Co-Investigator(Kenkyū-buntansha) |
TODA Shuji Saga Medical School, Faculty of Medicine, Assistant, 医学部, 助手 (80188755)
YONEMITSU Nobuhisa Saga Medical School, Faculty of Medicine, Associate Professor, 医学部, 助教授 (60145191)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Fat Tissue / Fat Cells / Proliferation / Small Fat Cells / Extracellular Matrix / Differentiation / Obesity / Adult Disease |
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| Research Abstract |
The proliferative ability of fat cells has been emphasized, both in the fat tissue in vivo and in the culture. We studied the proliferation of unilocular fat cells of young rats in a three-dimensional collagen gel matrix culture, which provides a physiological environment for fat cells. In this setting, the unilocular fat cells were able to maintain their cellular function and actively proliferate. In the culture of unilocular fat cells derived from 3 to 6-week-old rats in this study, very small fat cells appeared at the surface of a unilocular fat cell after the division of the nucleus. Thus, the small fat cells were born mostly in a "budding" manner and then proliferated. Insulin accelerated this process. In other unilocular fat cells, lipid droplets divided after the division of the nucleus, and very small fat cells appeared inside the unilocular fat cells. This was done in a "sharing" manner. Adenosine deaminase, a potent lipolytic factor, accelerated this process. An analysis of the cellular volume by a Coulter counter confirmed the birth of small fat cells from the unilocular fat cells. These small fat cells enlarged and turned into unilocular fat cells. It is supposed that not all unilocular fat cells are terminally differentiated, and do not lose their proliferative activity.
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Report
(3 results)
Research Products
(19 results)
