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ヒト・ホメオドメインタンパクによる新しいカドヘリン遺伝子の発現制御機構の解析

Research Project

Project/Area Number 06670240
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionTokai University School of Medicine

Principal Investigator

TANIGUCHI Yasushi  Tokai University School of Medicine, Department of nolec ular Life Science, Assistant Professor, 医学部, 講師 (30207188)

Co-Investigator(Kenkyū-buntansha) MORIUCHI Tetsuya  Hokkaido University , School of Medicine, Professor, 医学部, 教授 (20174394)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordshomeobox / HOXD3 / HEL cells / transfectants / expression vectors / integrin beta3 / R-cadherin / cell adhesion / インテグリンαIIbβ3
Research Abstract

We isolated the human homeobox gene HOXD3 on chromosome 2 from a human genomic library and determined its nucleotide sequence. In the present study, in order to study the role of the HOXD3 gene in erythropoiesis, expression vectors containing the HOXD3 gene inthe sense or antisense orientation were introduced into HEL cells. The sensetransfectants overexpressing the HOXD3 gene formed aggregates, which were composed of densely associated cells adhering to tissue-culture dishes, whereas the parental HEL cells and antisense transfectants adhered poorly to the dishes. Furthermore, the sense transfectants overexpressing the HOXD3 gene attached more efficiently to fibronectin and collagen than did the antisense transfectants and parental HEL cells. Northern blot analysis showed that integrin beta3 mRNA levels were significantly increased in the HEL cells overexpressing the HOX4A gene. FACS analysis showed that the sense transfectants expressed increased levelsof integrin alpha II bbeta3 complex. In addition, the antisense transfectants expressed R-cadherin more abundantly than the sense transfectants and parental HEL cells. These results implicate the homeobox gene HOXD3 in the regulation of cell abhesion processes.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Yoshimura S.: "The plasma glutathione peroxidase-encoding gene : organization,sequence and localization to chromosome 5q32." Gene. 145. 293-207 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Arimori K.: "Nucleotide sequence of a human genomic DNA fragment containing the PCNA pseudogene and its localization on chromosome 4." Tokai Exp.Clin.Med.19. 1-5 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Taniguchi Y.: "Overexpression of the HOX4A (HOXD3) homeobox gene in human erythroleukemia HEL cells results in altered adhesive properties." Blood. 85. 2786-2794 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshimura, S., Suemizu, H., Tani guchi, Y., Arimori, K., Kawabe, N., and Moriuchi, T.: "The plasma glutathione peroxidase-encoding gene : organization, sequence and localization to chromosome 5q3219GC01 : Gene" 145. 293-297 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Arimori, K., Koido, S., Yoshimura, S., Taniguchi, Y., Arimori, S., and Moriuchi, T.: "Nucleotide sequence of a human genomic DNA fragment containing the PCNA pseudogene and its localization on chromosome 4" Tokai Exp. Clin. Med.19. 1-5 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Taniguchi, Y., Komatsu, N., andMoriuchi, T.: "Overexpression of the HOX4A (HOXD3) homeobox gene in human erythroleukemia HEL cells results in altered adhesive properties" Blood. 85. 2786-2794 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Taniguchi, Y., Katsumata, Y., Koido, S., Suemizu, H., Yoshimura, S., Moriuchi, T., Okumura, K., Kagotani, K., Taguchi, H., imanishi, T., Gojobori, T., and Inoko, H.: "Cloning, sequencing, and chromosomal localization of two tandemly arranged human pseudogenes for the proliferating cell nuclear antigen (PCNA)." Genomics. (submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshimura,S.: "The plasma glutathione peroxidase-encoding gene : organization, sequence and localization to chromosome 5q32." Gene. 1994. 293-297 (145)

    • Related Report
      1995 Annual Research Report
  • [Publications] Arimori, K.: "Nucleotide sequence of a human genomic DNA fragment containing the PCNA pseudogene and its localization on chromosome 4." Tokai Exp. Clin. Med.19. 1-5 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Taniguchi, Y.: "Overexpression of the HOX4A (HOXD3) homeobox gene in human erythroleukemia HEL cells results in altered adhesive proerties." Blood. 85. 2786-2794 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoshimura,S.: "The human plasma glutathione peroxidase-encoding gene: organization,sequence and localization to chromosome 5g32." Gene. 145. 293-297 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Taniguchi,Y.: "Overexpression of the HOX4A (HOXD3) homeobox gene in human erythroleukemia HEL cells results in altered adhesive properties." Blood. (in press).

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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