| Project/Area Number |
06670246
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Tokyo Metropolitan institute of Gerontology |
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Principal Investigator |
MARUYAMA Naoki Tokyo Metropolitan Institute of Gerontology, Department of Molecular Pathology Head of Department, 分子病理, 研究部長 (00115940)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | human endogenous retrovirus / LTR / promoter / DNA binding protein / ヒト内在性レトロウイルス / 内在性レトロウイルス / クローン4-1 |
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| Research Abstract |
Human endogenous retrovirus genes are integrated in the chromosomal DNA and expressed by ordinal transcription factors.We investigated the promoter activity determined by LTR in human endogenous retrovirus gene Clone 4-1.By using luciferase assay as reporter gene and Jarkat cell we determined promoter fragment in the LTR of Clone 4-1.We determined the 184-bp fragment having promoter activity and 242-bp fragment having suppressive activity on the expression.We observed the presence of DNA binding protein to the 184-bp fragment by gel shift assay and in vivo foot printing assay.Our study suggested that the expression of human endogenous retrovirus gene is determined by a transcriptional element in LTR.Small numbers of DNA binding protein as transcriptional factors are involved in this process.
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