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Expression of human endogenous retrovirus gene in tumor cells

Research Project

Project/Area Number 06670246
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionTokyo Metropolitan institute of Gerontology

Principal Investigator

MARUYAMA Naoki  Tokyo Metropolitan Institute of Gerontology, Department of Molecular Pathology Head of Department, 分子病理, 研究部長 (00115940)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordshuman endogenous retrovirus / LTR / promoter / DNA binding protein / ヒト内在性レトロウイルス / 内在性レトロウイルス / クローン4-1
Research Abstract

Human endogenous retrovirus genes are integrated in the chromosomal DNA and expressed by ordinal transcription factors.We investigated the promoter activity determined by LTR in human endogenous retrovirus gene Clone 4-1.By using luciferase assay as reporter gene and Jarkat cell we determined promoter fragment in the LTR of Clone 4-1.We determined the 184-bp fragment having promoter activity and 242-bp fragment having suppressive activity on the expression.We observed the presence of DNA binding protein to the 184-bp fragment by gel shift assay and in vivo foot printing assay.Our study suggested that the expression of human endogenous retrovirus gene is determined by a transcriptional element in LTR.Small numbers of DNA binding protein as transcriptional factors are involved in this process.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Utunomiya, Y. 他: "Suppression of immune complex nephritis in NZB/WF_1 mice by a prostacycline analog." Clinical and Experimental Immunology. 99. 454-460 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujita, T. 他: "Isolation of cDNA clone encoding human senescencemarker protein-30(SMP30) and its location on the X chromosome" BBA. 1263. 249-252 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 関川〓 他: "間質性肺炎を伴った全身性エリテマトデス患者より分離されたレトロウィルスの解析" 日本炎症学会雑誌. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nagasawa, R., Maruyama, N., Kubo, S., Yumura, W., Mitarai, T., Isoba, K.and Kanagawa, O.: "Induction of Ly-49 on interspecies hybridoma between differentiation stage specific murine T cell and rat T lymphoma" Cellular Immunology. 156. 180-190 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kitamura, M., Maruyama, N., Shirasawa, T., Watanabe, K., Tateno, M.and Yoshiki, T.: "Expression of an endogenous retroviral gene product in human placenta." International J.Cancer. 58. 836-840 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Utsunomiya, Y., Ogura, M., Kawamura, T., Mitarai, T., Maruyama, N.and Sakai, O.: "Supperssion of immune complex nephritis in NZB/W F1 mice by a procycline analog." Clinical and Experimental Immunology. 99. 454-460 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sekigawa, I., Tamura, N., Hishikawa.T., Iida, N., Hashimoto, H., Shimizu, K., Hirose, S., Kira, S., Maruyama, N.and Yamamoto, N.: "Detection of type C retroviral particles in the lung cells of a patient with systemic lupus erythematosus (in Japanese)." Japanese J.Inflammation. 15. 401-408 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nagasawa, R.and Maruyama, N.: "Endogenous retrovirus and Autoimmunity (in Japanese)" Internal Medicine. 73. 640-644 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Maruyama, N: "Retrovirus and ageing (in Japanese)" Modern Chemistry. 24. 171-173 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sekigawa, I.and Maruyama, N.: "SLE and environmental factors-retroviral aspect-" lgaku-no-Ayumi. 176. 271-276 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Utsunomiya, Y.他: "Suppression of immune complex nephritis in N2B/WF_1 mice by a prostacycline analog." Clinical and Experimental Immunology. 99. 454-460 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fujita, T.他: "Isolation of cDNA clone encoding human senexence marker protein -30(SMP30) and its location on the X chromosome" BBA. 1263. 249-252 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 関川厳 他: "間質性肺炎を伴った全身性エリテマトーデス患者より分離されたレトロウイルスの解析" 日本炎症学会雑誌. (印刷中).

    • Related Report
      1995 Annual Research Report
  • [Publications] 関川厳 他: "SLEと環要因-レトロウイルスを中心として-"

    • Related Report
      1995 Annual Research Report
  • [Publications] 丸山直記 他: "ヒト内在性レトロウイルスと免疫疾患" Medical Immunology. 26. 155-160 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] 長沢龍司 他: "内在性レトロウイルスと自己免疫" 内科. 73. 640-644 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 丸山直記: "レトロウイルスと老化" 現代化学(増刊). 24. 171-173 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kitamura et al.: "Expression of an endogenaus retrouiral gene production in human placenta." International j.Cancer. 58. 836-840 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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