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Monoclonal growth during chimeric mouse carcinogenesis demonstrated by a strain-specific antibody and microsatellite DNA polymorphism patterns.

Research Project

Project/Area Number 06670250
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionAICHI CANCER CENTER

Principal Investigator

TATEMATSU Masae  Laboratory of Pathology, Aichi Cancer Center, Chief, 病理学第一部, 部長 (70117836)

Co-Investigator(Kenkyū-buntansha) MASUI Tsuneo  Laboratory of Pathology, Aichi Cancer Center, Senior Researcher, 病理学第一部, 主任研究員 (10190349)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsChimeric mouse / Colon / Forestomach / Anti C3H strain specific antigen antibody / PCR / SSCP / Clonal growth / Microsatellite DNA / Carcinogenesis / 大腸発癌 / 系統差
Research Abstract

The clonality of epithelial proliferative lesions associated with colon and forestomach carcinogenesis was immunohistochemically investigated in C3H/HeN**BALB/c chimeric mice using a specific antibody to C3H strain specific antigen (CSA) and as well as in terms of microsatellite DNA polymorphism patterns. In normal colonic mucosa of the chimeras, each gland was composed entirely of either CSA positive or negative cells and no mixed glands were found. Cells of all focal atypias in chimeric mice were, in each case, homogeneous for one or other of the parental types. Of 91 adenomas in chimeric mice, only one comprised both types of cells. Among 119 adenocarcinomas, 12 contained cells of both parental types. Normal chimeric forestomach epithelium was found to demonstrate mixtures of epithelial cell groups composed of either CSA positive or negative cells. The same was the case for all simple hyperplasias. Many PN-hyperplasias, in contrast, consisted of both CSA positive and negative cell groups. Squamous cell carcinomas were composed entirely of either CSA positive or negative tumor cells. However in animals with multiple advanced CSA positive cancers and negative cancers, cancer nests composed of both parental type cells were found in association. Microsatellite DNA ploymorphism patterns sampled from histological sections completely conformed with the outcome of immunohistochemical staining. The results suggest that individual cancers are derived from single cells showing monoclonal growth, with apparently polyclonal tumors arising secondarily during progression, due to two or more lesions coalescing.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] 立松正衛: "消化器癌のクローン性増殖" 血液・腫瘍科. 31. 433-441 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tatematsu, M.: "Primary monoclonal and secondary polyclonal growth of colon neoplastic lesions in C3H/Hen【tautomer】BALB/c chimeric mice treated with 1,2-dimethylhydrazine : Immunohistochemical detection of C3H strain-specific antigen and simple sequence lenth polymorphism analysis of DNA" Int. J. Cancer. (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tatematsu, M.: "Monoclonal development of squamous cell carcinomas from polyclonal papillary or nodular hyperplasias in the forestomach of C3H/HeN【tautomer】BALB/c chimeric mice treated with N-methyl-N-nitrosourea or diethylnitrosamine." Carcinogenesis. (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 立松正衛: "胃・大腸癌の単クローン増殖と細胞分化" 消化器癌. 6. 31-35 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tatematsu, M.: "Clonal growth of gastroenterological cancers." Hematology & Oncology. 31. 433-441 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tatematsu, M.: "Primary monoclonal and secondary polyclonal growth of colon neoplastic lesions in C3H/HeN**BALB/c chimeric mice treated with 1,2-dimethylhydrazine : Immunohistochemical detection of C3H strain-specific antigen and simple sequence lenth polymorphism analysis of DNA" Int.J.Cancer. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tatematsu, M.: "Monoclonal development of squamous cell carcinomas from polyclonal papillary or nodular hyperplasias in the forestomach of C3H/HeN**BALB/c chimeric mice treated with N-methyl-N-nitrosourea or diethylnitrosamine." Carcinogenesis. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tatematsu, M.: "Monoclonal growth and cellular differentiation of gastric and large intestinal cancers." Japanese Journal of Cancer of the Digestive Organs. 6. 31-35 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 立松正衛: "消化器癌のクローン性増殖" 血液・腫瘍科. 31. 433-441 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 立松正衛 et al.: "胃・大腸癌の単クローン増殖と細胞分化" 消化器癌. 6. 31-35 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tatematsu, M et al.: "Primary monoclonal and secondary polyclonal growth of colon neoplastic lesions in C31 HeN【tautomer】BALB/c chimeric treated with 1, 2-dimethylhydrazine: Immunohistochemic detection of C3H strain-specific antigen and simple sequence lenght polymorphism analys of DNA." Int. J. Cancer. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tatematsu, M. et al.: "Monoclonal development of squamous cell carcinomas from polyclonal papillary or nodular hyperplasias in the forestomach of C3H/Hen【tautomer】BALB/c chimeric mice treated with N-methyl-N-nitrosourea or diethylnitrosamine." Carcinogenesis. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tatematsu,M.,et al.: "Clonal analysis of glandular stomach carcinogenesis in C3H/HeN【tautomer】BALB/c chimeric mice treated with N-methy1-N-nitrosourea." Cancer Lett.83. 37-42 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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