| Project/Area Number |
06670272
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
寄生虫学(含医用動物学)
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| Research Institution | Tokai University School of Medicine |
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Principal Investigator |
NAGAKURA Koichi Tokai University School of Medicine, Department of Infectious Diseases, Assistant Professor., 医学部, 講師 (80142454)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | INTESTINAL PARASITES / SEROTONIN / DIARRHEA / SEROTONIN TRANSPORTER / Entamoeba histolytica / Giardia lamblia / INDOLEACETIC ACID |
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| Research Abstract |
Parasitic protozoa take the require materials and excludes themetabolic products through the transporter proteins of the plasma membrane. Mac-Gawan (1988) reported that the diarrhea, that observed frequently at the infection of intestinal protozoa, causes by the secretion of serotonin (5HT) from intestinal protozoa. The aim of this project is the confirmation and of the functions of the serotonin the molecular identification of serotonin transporter in the plasma membrane.
The trophozoties of Entamoeba histolytica and Giardia lamblia were not stained immunohistochemically using human anti-serotonin antibody. And the culture media and the homogenates of parasites were not contained the serotonin, but indoleacetic acid were detected. Moreover, no ampli- fied products obtained by polymerase chain reaction using multiple primers for mammalian transporter. These results suggested that parasitic protozoa secreated not serotonin by the way of transporter. On the other hand, the specific antibody against mammalian transporter protein was detected in the sera of the patients with parasitic diseases regardless protozoa and helminth infection. It is seem to be that the antibody is the protein occurred in the acute phase of the infection.
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