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Experimental analysis of symbiosis of Orientia tsutsugamushi and Leptorombidium.

Research Project

Project/Area Number 06670275
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 寄生虫学(含医用動物学)
Research InstitutionNiigata College of Pharmacy

Principal Investigator

URAKAMI Hiroshi  Niigata College of Pharmacy, Associate Professor, 薬学部, 助教授 (80139732)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsOrientia tsutsugamushi / rickettsia / scrub typhus / intracellular symbiosis / Leptotrombidium / sex ratio / serotype / vertical transmission / rickettsia / モノクローナル抗体 / Rickettsia tsutsugamushi / 共生 / 顕微注射
Research Abstract

We analyzed the symbiosis of Orientia tsutsugamushi and Trombiculid mite by microbiological and experimentaltechniques.
1.We developed a method to observe rickettsiae in the mites by immunofluorescent microscopy, which largely improved a laborious work to maintain rickettsia-positive colonies of Leptotrombidium.
2.Secrotype of the rickettsiae in a single mite could be analyzed by the above-mentioned method with type-specific monoclonal antibodies. It was demonstrated that each mite and each family line of the mites harbor rickettsiae of a single serotype. This is the contrary to an opinion so far claimed.
3.To know the reason why the colony of O.tsutsugamushi-harboring L.fletcheri consists only from females, unfed larvae in the colony were fed on laboratory mice which had been injected with minocycline, a rickettsiacidal antibiotic agent. The rickettsiae in the mites were successfully eliminated from the mites, and sex ratio was observed in the descendants. All the mites at the P and F1 generations were females, but males appeared and dominated at F2 through F5 generations. This suggests that the rickettsia causes female-biased sex ratio in the rickettsia-harboring colony of L.fletcheri.
4.Strains of O.tsutsugamushi isolated isolated in Japan were analyzed by monoclonal antibodies and RFLP of a gene cording the strain specific antigen protein.It was indicated that strains in each serotype of O.tsutsutgamushi could be further classified into subtypes.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 浦上 博,多村 憲: "恙虫病リケッチアOrientia tsutsugamushiと宿主ツツガムシとの共生関係について" 日本細菌学雑誌. 51. 497-511 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N.Ohashi,Y.Koyama,H.Urakami,et al.: "Demonstration of antigenic and genotypic variation in Orientia tsutsugamushi which were isolated in Japan,and their classification into type and subtype." Microbiol.Immunol.40. 627-638 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Takahashi,H.Urakami,et al.: "Occurrence of high ratio of males after introduction of minocycline in a colony of Leptotrombidium fletcheri infected with Orientia tsutsugamushi." Eur.J.Epidemiol.13. 79-86 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kawamura,A.Jr.,Tanaka,H.,and Tamura,A.eds.: "Tsutsugamushi disease" University of Tokyo Press, 362 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Urakami, A.Tamura: "Symbiotic relationship between Orientia tsutsugamushi and Trombiculid mites." Jpn.J.Bacteriol.51. 497-511 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N.Ohashi, Y.Koyama, H.Urakami, et al.: "Demonstration of antigenic and genotypic variation in Orientia tsutsugamushi which were isolated in Japan, and their classification into type and subtype." Microbiol.Immunol. 40. 627-638 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Takahashi, H.Urakami, et al.: "Occurence of high ratio of males after introduction of minocycline in a colony of Leptotrombidium fletcheriinfected with Orientia tsutsugamushi." Eur.J.Epidemiol.13. 79-86 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A.Tamura, H.Urakami: Microbiological aspects of Rickettsia tsutsugamushi Kawamura, A.Jr.et al.eds., Tsutsugamushi disease.University of Tokyo Press, 35-90 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A.Tamura, H.Urakami: Detection Rickettsia tsutsugamushi in mites. Kawamura, A.Jr.et al.eds., Tsutsugamushi disease.University of Tokyo Press, 239-246 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A.Tamura, H.Urakami: Transmission dynamics of Rickettsia tsutsugamushi : Transmission from mammals to mites. Kawamura, A.Jr.et al.eds., Tsutsugamushi disease.University of Tokyo Press, 267-274 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tamura, A., Ohashi, N., Urakami, H., Miyamura, S.: "Classification of Rickettsia tsutsugamushi in a New Genus, Orientia gen. Nov., as Orientia tsutsugamushi comb. nov." Intern. J. System. Bacteriol.45. 589-591 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 浦上 弘、多村 憲: "恙虫病リケッチアOrientia tsutsugamushiと宿主ツツガムシとの共生関係について" 日本細菌学雑誌. (印刷中).

    • Related Report
      1995 Annual Research Report
  • [Publications] Takahashi, M. Urakami, H. et al.: "Occurrence of high ratio of males after introduction of minocycline in a colony of Leptotrombidium fletcheri infected with Rickettsia tsutsugamushi." Europ. J. Epidemiol.(印刷中).

    • Related Report
      1995 Annual Research Report
  • [Publications] Kawamura, A., Jr., Tanaka, H. Tamura, A., eds.: "Tsutsugamushi Disease" University of Tokyo Press, 362 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Urakami,H.;Takahashi,M.Hori,E.;Tamura,A.: "An ultrastructural study of vertical transmission of R.tsutsugamushi during oogenesis and spermatogenesis in Leptotrombidium pallidum." Am J Trop Med Hyg. 50. 219-228 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Urakami,H.;Takahashi,M.Murata,M.;and Tamura,A.: "Electron microscopic study of the distribution and the vertical transmission of Rickettsia tsutsugamushi in Leptotrombidium pallidum." Jpn.J.Med.Sci.Biol.47. 127-139 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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