|Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
In this report we studied on the mechanisms responsible for intraerythrocytic growth of P.falciparum and present the following data :
1)A commercial preparation, Daigo's GF21 derived from adult bovine serum, developed as a growth-promoting factor for many kinds of mammalian cells was found to sustain growth of the parasite when T basal medium was employed as a basal medium.The optimal Daigo's GF21 concentration for parasite growth was between 5-20%, with the best results at 10%.differential counts indicated that the growth factor is essential for schizogony.Establishied serum-free medium, GIT,containing the factor yielded good parasite growth without any supplementation.
2)A low molecular weight factor in a basal medium essential for erythrocytic P.falciparum development in a serum-free medium using a cell-growth-promoting factor derived from adult bovine serum was detected.The factor was hypoxanthine.The optimal hypoxanthine concentration for parasite growth was between 15-120 muM.Among various low molecular weight supplements tested, adenine, adenosine, AMP,ATP,cyclic AMP,guanine, guanosine, inosine, inosine monophosphate, xanthine, NAD,NADH,NADP,NADPH and deoxyguanosine triphosphate showed a similar effect to that of hypoxanthine in the serumfree culture system.