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Identification of a novel protectiveantigen of Mycobacterium bovis and its application to the host defense

Research Project

Project/Area Number 06670285
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Bacteriology (including Mycology)
Research InstitutionNiigata University

Principal Investigator

KAWAMURA Ikuo  Niigata University School of Medicine, Assistant Professor, 医学部, 助手 (20214695)

Co-Investigator(Kenkyū-buntansha) MITSUYAMA Masao  Niigata University School of Medicine, Professor, 医学部, 教授 (10117260)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsMycobacterium / Host defense / Protective antigen / Protective immunity / Vaccine / ワクチン / Mycobacterium bovis BCG / 感染抵抗性T細胞 / BCG菌体抗原 / IFNγ
Research Abstract

The purpose of this study was to identify the protective antigen of Mycobacterium bovis BCG,which is critically important for the induction of IFN-gamma-producing protective CD4^+ T cells, and to determine the importance of this protective antigen in the expression of anti-tuberculosis immunity. The following results were obtained.
1) In mice immunized with viable BVG,IFN-gamma-producing T cells were induced along with the protective immunity. In contrast, neither such T cells nor protective immunity could not be induced when immunization was done by killed BCG,though DTH reaction was generated. Among several fractions prepared from BCG cells, a 18 kDa antigen exhibited a marked ability to stimulate IFN-gamma production of BCG-immune spleen cells. This particular antigen was shown to be present not only in viable BCG or PPD but also in killed cells of BCG,suggesting that some factor other than antigen is involved in the induction of protective immunity of the host.
2) T cell proliferatio … More n and IL-2 production were observed in BCG immune cells against a wide range of antigens with varying molecular weights, suggesting the insignificance of these paramenters as the establishment of protective immunity.
3) We have determined the cytokine response of normal spleen cells after stimulation with viable or killed BCG.TNF-alpha expression was induced equally by both viable and killed BCG,while NO production was induced only by viable BCG.Viable cells of BCG was capable of inducing IFN-gamma in NK cells but killed BCG was not. NK cell-derived IFN-gamma appeared to be indispensable for iNOS expression. The difference of NO-inducing ability between viable and killed BCG seemed to be depending on the ability to induce IFN-gamma from NK cells. Considering the importance of IFN-gamma in the functional differentiation of Th1 type cells, it was suggested that the inability of killed BCG vaccine in the induction of protective immunity was attributable to the lack of IFN-gamma induction at the early stage of immunization. Less

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Xiong,Huabao: "Cytokine gene expression in mice at an early stage of infection with various strains of Listeria spp. differing ib virulence." Infection and Immunity. 62. 3649-3654 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kawamura,Ikuo: "Antigen provoking g-IFN production in response to Mycobacterium bovis BCG and functional difference in T cell response to this antigen between viable and killed BCG-immunized mice." Infection and Immunity. 62. 4396-4403 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Oshikiri,Kuninaka: "Specific immune response to the 40kDa outer membrane protein of Porphyromonas gingivalis in mice." Arcives of Oral Biology. 39. 707-713 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yasumoto,Shin-ichiro: "Ultraviolet-B- irradiation alters cytokine production by immune lymphocytes in herpes simplex virus-infected mice." Journal of Dermatological-Science. 8. 218-223 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nishibori,Takeaki: "Correlation between the presence of virulence-associated genes as determined by PCR and actual virulence to mice in various strains of Listeria spp." Microbiology and Immunology. 39. 343-349 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yang,jianfei: "Involvment of natural killer cells in nitric oxide production by spleen cells stimulated with Mycobacterium bivis BCG." Journal of Immunology. 155. 5728-5735 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 光山 正雄(分担): "細菌感染の分子医学-その新展開-(渡辺治雄 編)" 羊土社, 178 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Xiong, Huabao: "Cytokine gene expression in mice at an early stage of infection with various strains of Listeria spp. differing in virulence." Infection and Immunity. 62. 3649-3654 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kawamura, Ikuo: "Antigen provoking gamma-IFN production in response to Mycobacterium bovis BCG and functional difference in T-cell responses to this antigen between viable and killed BCG-immunized mice." Infection and Immunity. 62. 4396-4403 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Oshikiri, Kuninaka: "Specific immune response to the 40 kDa outer membrane protein of Porphyromonas gingivalis in mice." Archives of Oral Biology. 39. 707-713 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yasumoto, Shin-ichiro: "Ultraviolet-B irradiation alters cytokine production by immune lymphocytes in herpes simplex virus-infected mice." Journal of Dermatological Science. 8. 218-223 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nishibori, Takeaki: "Correlation between the presence of virulence-associated genes as determined by PCR and actual virulence to mice in various strains of Listeria spp." Microbiology and Immunology. 39. 343-349 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yang, Jianfei: "Involvement of natural killer cells in nitric oxide production by spleen cells stimulated with Mycobacterium bovis BCG." Journal of Immunology. 155. 5728-5735 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Xiong,Huabao: "Cytokine gene expression in mice at an early stage of infection with various strains of Listeria spp. differing in virulence." Infection and Immunity. 62. 3646-3654 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kawamura,Ikuo: "Antigen provoking g-IFN production in response to Mycobacterium bovis BCG and functional difference in T cell response to this antigen between viable and killed BCG-immunized mice." Infection and Immunity. 62. 4396-4403 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Oshikiri,Kuninaka: "Specific immune response to the 40 kDa outer membrane protein of Porphyromonas gingivalis in mice." Archives of Oral Biology. 39. 700-713 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yasumoto,Shin-ichiro: "Ultraviolet-B irradiation alters cytokine production by immune Lymphocytes in herpes simplex virus-infected mice." Journal of Dermatological Science. 8. 218-223 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nishibori,Takeaki: "Correlation between the presence of virulence-associated genes as determined. by PCR and actual virulence to mice in various strains of Listeria spp." Microbiology and Immunology. 39. 343-349 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yang,Jianfei: "Involvment of natural killer cells in nitric oxide production by spleen cells stimulated with Mycobacterium bovis BCG" Journal of Immunology. 155. 5728-5735 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 光山正雄: "細菌感染の分子医学-その新展開(渡辺治雄編)" 羊土社, 178 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ikuo Kawamura: "Antigen provoking gamma interferon production in response to Mycobacterium bovis BCG and functional difference in T-cell responses to this antigen between viable and ...." Infection and Immunity. 62. 4396-4403 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Huabao Xiong: "Cytokine gene expression in mice at an early stage of infection with various strains of Listeria spp.differing in virulence" Infection and Immunity. 62. 3649-3654 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shinichiro Yasumoto: "Ultraviolet-B irradiation alters cytokine production by immune lymphocytes in herpes simplex virus -infected mice" Journal of Dermatological Science22GD03:8. 218-223 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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