| Project/Area Number |
06670288
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Nagoya University |
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Principal Investigator |
ARAKAWA Yoshichika Nagoya Univ., Bacteriology, Associate Prof., 医学部, 助教授 (10212622)
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| Co-Investigator(Kenkyū-buntansha) |
OHTA Michio Nagoya Univ., Bacteriology, Professor., 医学部, 教授 (20111841)
ITO Hideo Nagoya Univ., Medical Technology, Associate Prof., 医療技術短期大学部, 助教授 (90144162)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Klebsiella pneumoniae / capsular polysaccharide / Gene organization / Klebsiella pneumoniae / クレブシエラ / 多糖合成遺伝子 / 糖転移酵素 / 病原因子 / 塩基配列 / ポリシストロン |
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| Research Abstract |
In these two years, we have been studying on the biosynthesis mechanisms of bacterial capsular polysaccharide (CPS) which has been regarded as one of the bacterial pathogenic determinants. The cps gene cluster of K pneumoniae Chedid (K2 : O1), which is involved in the biosynthesis of serotype K2 CPS,has been subcloned into M13 phages, mp 18 and mp 19, and the entire nucleotide sequence, about 24-kb, was determined in the term of this project. This investigation provided us many important new findings and information about the structure of the Klebsiella cps cluster and its regulation. At least 19 putative open reading frames (ORFs) were identified in the sequenced area, and several genes similar to the cpsG,cpsB,gnd, and galF were found in this region. The region implicated in the synthesis of serotype K2 CPS was localized in the central portion of the sequenced area, and it was suggested that the expression of genes in this region still continue even in the stationary phase by the function of a gene locating in the upstream region of the ORF3. However, the functions of several genes are still unknown, therefore we are planning to continue the analyzes of their roles in the biosynthesis of CPS in the next term of investigation.
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