• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Role of intestinal bacteria in small intestinal ulcer formation and prevention in rats treated with a nonsteroidal antiinflammatory drug.

Research Project

Project/Area Number 06670296
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Bacteriology (including Mycology)
Research InstitutionThe University of Tokushima

Principal Investigator

OHNISHI Yoshinari  The University of Tokushima, School of Medicine, Professor, 医学部, 教授 (10037400)

Co-Investigator(Kenkyū-buntansha) KATAOKA Keiko  The University of Tokushima, School of Medicine, Research associate, 医学部, 教務員 (40189303)
KINOUCHI Takemi  The University of Tokushima, School of Medicine, Lecturer, 医学部, 講師 (80136217)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsNonsteroidal antiinflammatry drug / Small intestinal ulcer / Intestinal bacteria / Germ-free rats / Gnotobiotes / Lactobacillus acidophilus / Bifidobacterium adolescentis / Escherichia coli
Research Abstract

This study examined the role of intestinal bacteria in induction and repression of ulcer formation in the ileum of rats treated with one of the nonsteroidal antiinflammatry drugs (NSAIDs), 5-bromo-2-(4-fluorophenyl)-3-(4-methylsulfonylphenyl) thiophene (BFMeT). BFMeT was administered by intragastric gavage once at doses of 500-1500 mg/kg of body weight in a volume of 10 ml/kg to Wistar rats treated with and without antibiotics (bacitracin, neomycin, streptomycin), germ-free rats and gnotobiotic rats, and 72 hours later their gastrointestinal tracts were examined for ulcer formation. Single oral administration of BFMeT induced ileal ulcers in specific pathogen-free rats. However, the rats given antibiotics to reduce the intestinal bacteria had no ulcers.BFMeT-treated germ-free rats and gnotobiotic rats mono-associated with Bifidobacterium adolescentis or Lactobacillus acidophilus also had no intestinal ulcers. However, the drug induced ileal ulcers in gnotobiotic rats mono-associated wi … More th Eubacterium limosum or Escherichia coli. The number of viable microorganisms in the ulcerated ileum was 13.6-fold higher in the aerobic culture and 8.8-fold higher in the anaerobic culture when compared with that in the normal rats. An overnight culture of B.adolescentis or L.acidophilus or yogurt containing Bifidobacterium breve and Streptococcus thermophilus, when given as drinking water, inhibited the ulcer formation in the ileum of rats treated with BFMeT.Gram staining of the ileal contents of normal rats revealed that 97.4% of the stained microorganisms were Gram-positive rods and only 1.2% were Gram-negative rods. In the group of rats with ulcers induced by BFMet, the Gram-positive rods decreased by 56.4% and the Gram-negative rods including Escherichia coli, Klebsiella, Proteus and Bacteroides increased by 37.3%. However, in the group of rats administered the Bifidobacterium culture, the Lactobacillus culture or yogurt, percentages of the Gram-negative rods were decreased. Although Lactobacillus was a major bacterium in the ileum of normal rats, the Gram-negative facultatively anaerobic rods Escherichia coli, Klebsiella and Proteus were increased in the ulcerated ileum of rats treated with BFMeT,suggesting that these bacteria are associated with the ulcer formation in rats treated with NSAIDs, and that Lactobacillus and Bifidobacterium inhibit it by repressing the growth of ulcer-inducing bacteria. Less

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] T.Kinouchi,K.Kataoka,Y.Ohnishi et al.: "Biological activities of the intestinal microflora in mice treated with antibiotics or untreated and the effects of the microflora on absorption and metabolic activation of orally administered glutathione conjugates of K-region epoxides of 1-nitropyrene." Carcinogenesis,. 14,. 869-874 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Kataoka,T.Kinouchi,S.Akimoto and Y.Ohnishi: "Bioactivation of cysteine conjugates of 1-nitropyrene oxides by cysteine conjugate β-lyase purified from Peptostreptococcus magnus." Appl.Environ.Microbiol.,. 61,. 3781-3787 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Uejima,T.Kinouchi,Y.Ohnishi et al.: "Role of intestinal bacteria in ileal ulcer formation in rats treated with a nonsteroidal antiinflammatory drug." Microbiol Immunol.,. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 木内武美、大西克成 他: "BFMeTが誘発するラット小腸潰瘍形成および腸内菌叢の変化に及ぼすL.acidophilusおよびB.adolescentisの培養菌液投与の影響" (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Kinouchi, K.Kataoka, K.Miyanishi, S.Akimoto and Y.Ohnishi: "Biological activities of the intestinal microflora in mice treated with antibiotics or untreated and the effects of the microflora on absorption and metabolic activation of orally administered glutathione conjugates of K-region epoxides of 1-nitropyrene." Carcinogenesis. 14. 869-874 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Kataoka, T.Kinouchi, S.Akimoto and Y.Ohnishi: "Bioactivation of cysteine conjugates of 1-nitropyrene oxides by cysteine conjugate beta-lyase purified from Peptostreptococcus magnus." Appl.Environ.Microbiol.61. 3781-3787 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Motoo Uejima, Takemi Kinouchi, Keiko Kataoka, Isao Hiraoka and Yoshinari Ohnishi: "Role of intestinal bacteria in ileal ulcer formation in rats treated with a nonsteroidal antiinflammatory drug." Microbiol Immunol.(To be submitted). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takemi Kinouchi, Keiko Kataoka, Motoo Uejima and Yoshinari Ohnishi: "Effect of bacterial cultures of L.acidophilus and B.adolescentis on formation of BFMeT-induced small intestinal ulcer and intestinal frola modification." (In preparation).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T. Kinouchi, K. Kataoka, Y. Ohnishi et al.: "Biological activities of the intestinal microflora in mice treated with antibiotics or untreated and the effects of the microflora on absorption and metabolic activation of orally administered glutathione conjugates of K-region epoxides of l-nitropyrene." Carcinogenesis,. 14. 869-874 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] K. Kataoka, T. Kinouchi, S. Akimoto and Y. Ohnishi: "Bioactivation of cysteine conjugates of l-nitropyrene oxides by cysteine conjugate β-lyase purified from Peptostreptococcus magnus." Appl. Environ. Microbiol.61. 3781-3787 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M. Uejima, T. Kinouchi, Y. Ohnishi et al.: "Role of intestinal bacteria in ileal ulcer formation in rats treated with a nonsteroidal antiinflammatory drug." Microbiol Immunol.(発表予定).

    • Related Report
      1995 Annual Research Report
  • [Publications] 木内武美,大西克成 他: "BFMeTが誘発するラット小腸潰瘍形成および腸内菌叢の変化に及ぼすL.acidophilusおよびB.adolescentisの培養菌液投与の影響" (発表予定).

    • Related Report
      1995 Annual Research Report
  • [Publications] 大西克成: "腸内菌の代謝" ヘルシスト. 17(3). 64-69 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 大西克成・木内武美: "腸内菌の働き" New Food Industry. 37(印刷中)-(3月号). (1995)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi