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Molecular mechanism of measles virus-induced immunosuppression

Research Project

Project/Area Number 06670322
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionKyusyu University (1995)
The University of Tokyo (1994)

Principal Investigator

YANAGI Yusuke  Kyusyu University, Medicine Professor, 医学部, 教授 (40182365)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsMeasles virus / Immunosuppression / CD46 / 麻疹ウイルスレセプター / 細胞融合
Research Abstract

Mouse fibroblast cell lines (L, NIH3T3) supported a low level of measles virus (MV) replication. These fibroblasts never formed multinucleated giant cells, a characteristic cytopathic effect of MV infection. We derived L cell transfectants stably expressing the gene encoding the human CD46, the recently identified MV receptor. Upon infection with MV, these transfectants produced 20 fold more MV, became 100 time more sensitive to MV than the parental L cells, and developed syncytia. To determine the domain (s) in CD46 essential for the receptor function, several deletion mutants were generated that lacked the cytoplasmic region or each one of the four short consensus repeat (SCR) of CD46. Mutants lacking SCR1 or SCR2 were found not to act as the receptor, indicating that SCR1 or SCR2 are indispensable for the function as the MV receptor. On the basis of these results, we produced transgenic mice expressing the human CD46 as an animal model to study immunosuppression observed during MV infection. We are currently testing whether these mice are susceptible to MV, and develop any clinical signs.
We also examined the mechanism by which MV inhibitits thein vitro proliferation of T cells. Coculture with cells expressing MV glycoproteins without producing infectious MV did not lead to the inhibition of the mitogen-induced T cell proliferation, indicating that the cell-cell interaction through MV glycoproteins expressed on MV-infected cells and surface molecules on uninfected cells is not sufficient to cause the inhibition of T cell proliferation.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Yanagi, Y.: "Measles virus infects mouse fibroblast cell lines, but its multiplication is severely restricted in the absence of CD46." Archives of Virology. 138. 39-53 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sakae, H.: "L cell clone developing plaques upon infection with measles virus (Edmonston strain)." Archives of Virology. 139. 427-430 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seya, T.: "Blocking measles virus infection with a recombinant soluble form of, or monoclonal antibodies against, membrane cofactor protein of comprement (CD46)." Immunology. 84. 619-625 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Iwata, K.: "Diversity of sites for meales virus binding and for inactivation of complement C3b and C4b on membrane cofactor protein CD46." The Journal of Biological Chemistry. 270. 15148-15152 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 柳 雄介: "麻疹ウイルスの感染および免疫抑制機構" ウイルス. 45. 117-123 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 柳 雄介: "麻疹ウイルスレセプター, CD46" ウイルス. 45. 159-164 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yanagi, Y.et al.: "Measles virus infects mouse fibroblast cell lines, but its multiplication is severely restricted in the absence of CD46" Archives of Virology. 138. 39-53 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sakae, H.et al.: "L cell clone developing plaques upon infection with measles virus (Edmonston strain)" Archives of Virology. 139. 427-430 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seya, T.et al.: "Blocking measles virus infection with a recombinant soluble from of, or monoclonal antibodies against, membrane cofactor protein of complement (CD46)" Immunology. 84. 619-625 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Iwata, K.et al.: "Diversity of sites for measles virus binding and for inactivation of complement C3b and C4b on membrane cofactor protein CD46" Journal of Biological Chemistry. 270. 15148-15152 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yanagi, Y.: "Measles virus infection and Immunosuppression (in Japanese)" Virus. 45.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 1995: "117-123" Yanagi, Y.Measles virus receptor CD46 (in Japanese).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yanagi, Y.: "Measles virus infects mouse fibroblast cell lines, but its multiplication is severely restricted in the absence of CD46." Archives of Virology. 138. 39-53 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sakae, H.: "L cell clone developing plaques upon infection with measles virus (Edmonston strain)." Archives of Virology. 139. 427-430 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Seya, T.: "Blocking measles virus infection with a recombinant soluble form of, or monoclonal antibodies against, membrane cofactor protein of comprement (CD46)." Immunology. 84. 619-625 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Iwata, K.: "Diversity of sites for meales virus binding and for inactivation of complement C3b and C4b on membrane cofactor protein CD46." The Journal of Biological Chemistry. 270. 15148-15152 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 柳雄介: "麻疹ウイルスの感染および免疫抑制機構" ウイルス. 45. 117-123 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 柳雄介: "麻疹ウイルスレセプター,CD46" ウイルス. 45. 159-164 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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