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Mode of replication and pathogenicity of human herpesvirus 7

Research Project

Project/Area Number 06670329
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionOkayama University

Principal Investigator

YAMADA Masao  Okayama Univ., Medical Sch., Associate Professor, 医学部, 助教授 (40166731)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Jun  Okayama Univ., Medical Sch., Research Associate, 医学部, 助手 (20227903)
NII Shiro  Okayama Univ., Medical Sch., Professor, 医学部, 教授 (40029757)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsHuman herpesvirus 7 / Human herpesvirus 6 / Virus replication / Pathoqenicity / Persistent infection / Interferon / Human Immunodeficiency virus / CD4 / Tリンパ球 / ヒト免疫不全ウイルス
Research Abstract

Human Herpesvirus 7 (HHV-7), a new T-lymphotropic herpesvirus, is related to, but significantly different from HHV-6. We isolated several clinical isolates from saliva samples of healthy adults, and they were identified as HHV-7. We established a method for infectious titration and a method to obtain high titer cell-free virus preparation using cord blood lymphocytes. One-step growth curves of HHV-6A,6B,and 7 were comparatively analyzed. Among 21 hematopoietic cell lines, a CD4+ CD8+ T cell line, SUP-T1, was the only cell line that supported HHV-7 replication. Virus titers of HHV-7 in SUP-T1 cells were comparable to those in cord blood lymphocytes.
Persistent production of infectious virus was observed after infection of SUP-T1 cells with HHV-7. The culture was the mixture of infected and uninfected cells with the ratio of the infected cells ranging around 10 to 20%. Interferon activity was not detected in the supernatant. However, the treatment of the culture with exogenous interferon beta dramatically reduced the production of virus and cured the culture of the persistent infection. No virus production, infected cell, or HHV-7 genome was detectable.
We studied the interaction of HIV-1 and HHV-7 using the SUP-T1 cell line and a HIV carrier cell line established from SUP-T1. Expression of the CD4 antigen was completely downregulated in the HIV-1 carrier cell line. While superinfection of HIV-1 carrier SUP-T1 cells with HHV-6 was readily accomplished, the same cells were completely resistant to superinfection with HHV-7. Furthermore, treatment of SUP-T1 cells with anti-CD4 monoclonal antibodies inhibited the growth of HHV-7. These results suggest that resistance of HIV-1 carrier SUP-T1 cells to HHV-7 is due to the absence of the CD4 antigen on the cell surface and that HIV-1 and HHV-7 use the same molecule, the CD4 antigen, as the virus receptor.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Yamada M: "Resisutance of HIV-1 carrier SUP-T1 cells to superinfection with human herpesvirus7." AIDS Research Newsletter. 37 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hayashi K: "HTLV-ll non-integrated malignant lymphoma induction in japanese white rabbits following intravenous inoculation of HTLV-ll-infected simian leukocyte cell line(Si-IIA)." Japanese Journal of Cancer Research. 85. 808-818 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hayashi K: "Malignant lymphoma induction in rabbits by intravenous inoculation of Epstein-Barr virus-related herpesvirus from HTLV-II-transformed cynomolgus luekocyte cell line (Si-ILA)." International Journal of Cancer. 63. 872-880 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nakamura J: "Analysis of molluscum contagiosum virus genomes isolated in Japan." Journal of Medical Virology. 46. 339-348 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hayase Y: "Ultrahigh-resolution scanning electron microscopy of MDCK cells infected with influenza viruses." Journal of Electron Microscopy. 44. 281-288 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshida M: "A simple method for detection of HHV-6 antigens and its utilization for evaluating antiviral drugs." Journal of Virological Methods. (印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Muraki Y: "Electron microscopic observation of aberrent capsids of pseudorabies virus." Journal of Electron Microscopy. (印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 平松 祐司: "サイトメガロウイルス感染症の出世前診断-症例と診断上の問題点-" 産婦人科の実際. 44. 919-924 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamada M: "Resistance of HIV-1 carrier SUP-T1 cells to superinfection with human herpesvirus 7" AIDS Research Newsletter. 37. (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hayashi K: "HTLV-II non-integrated malignant lymphoma induction in japanese white rabbits following intravenous inoculation of HTLV-II-infected simian leukocyte cell line (Si-IIA)." Japanese.Journal of Cancer Research. 85. 808-818 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hayashi K: "Malignant lymphoma induction in rabbits by intravenous inoculation of Epstein-Barr virusrelated herpesvirus from HTLV-II-transformed cynomolgus leukocyte cell line (Si-IIA)" International Journal of Cancer. 63. 872-880 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nakamura J: "Analysis of molluscum contagiosum virus genomes isolated in Japan." Journal of Medical Virology. 46. 339-348 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hayase Y: "Ultrahigh-resolution scanning electron microscopy of MDCK cells infected with influenza viruses." Journal of Electron Microscopy. 44. 281-288 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshida M: "A simple method for detection of HHV-6 antigens and its utilization for evaluating antiviral drugs." Journal of Virological Methods. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Muraki Y: "Electron microscopic observation of aberrent capsids of pseudorabies virus." Journal of Electron Microscopy. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiramatsu Y: "Pretrnatal diagnosis of cytomegalovirus infection : Case reports and key for diagnosis (in Japanese)" Sanfujinnka-no-jissai. 44(7). 919-924 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hayashi K: "Malignant Iymphoma induction inrabbits by intravenous inoculation of Epstein-Barr virus-related herpesvirus from HTLV-II-transformed cynomolgus leukocyte cell line (Si-IIA)." International Journal of Cancer. 63. 872-880 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nakamura J: "Analysis of molluscum contagiosum virus genomes isolated in Japan." Journal of Medical Virology. 46. 339-348 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoshida M: "A simple method for detection of HHV-6 antigens and its utilization for evaluating antiviral drugs." Journal of Virologcal Methods. 印刷中. (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hayase Y: "Ultrahigh-resolution scanning electron microscopy of MDCK cells infected with influenza viruses." Journal of Electron Microscopy. 44. 281-288 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 平松祐司.: "サイトメガロウイルス感染症の出世前診断-症例と診断上の問題点-" 産婦人科の実際. 44. 919-924 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yamada M.: "Resistance of HIV-lcarrier SUP-T1 cells to superinfection with human herpesvirus 7." AIDS Research Newsletter. 37. (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hayashi K.: "HTLV-II non-integrated malignant lymphoma induction in Japanese white rabbits following intravenous inoculation of HTLV-II-infected simian leukocyte cell line(Si-IIA)" Jap.J Cancer Res.86(8). 808-818 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 中村 純: "伝染性軟属腫のウイルス学" メディア サークル. 39(9). 301-307 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 新居志郎: "単純ヘルペスウイルスのin vitro持続感染系に及ぼすインターフェロン投与の影響" インターフェロン-βの研究-その現在と新たな展開-. 64-65 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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