| Project/Area Number |
06670389
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hygiene
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| Research Institution | Iwate Medical University |
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Principal Investigator |
ITAI Kazuyoshi Iwate Medial University, Hygiene & Pub.Health, Lecturer, 医学部, 講師 (10048572)
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| Co-Investigator(Kenkyū-buntansha) |
ONODA Toshiyuki Iwate Medial University, Hygiene & Pub.Health, Research associate, 医学部, 助手 (00254748)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Osteoporosis / Bone density / Fluoride ion concentration in serum |
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| Research Abstract |
The method for the determination of a very small amount of fluorid ion in serum was developed. In this method, a flow injection apparatus with a fluoride ion-selective electrode as a detector was used. A severe interference by phsphate ion was investigated, when a fluoride concentration of a serum sample was determined using this apparatus. To restrain this interference, proper amount of phosphte ion was added to the buffer solution. In the practical measurement, a quantity of addition of phosphate ion was selected as 0.1M/L.0.03M/L of CyDTA was added in the buffer solution to restrain the interference by calcium ion. The value of pH of the buffer solution was decided in 5.3. The pH value was adjusted in 5.0-5.3 after having diluted a serum sample with distillled water, and the recovery was investigated. When it was diluted in 10 times, the recovery of 96.5% was obtained.
72 serum specimens were selected among the women who measured bone density using DEXA apparatus, and the fluoride ion concentrations was measured. The mean <plus-minus> standard deviation was 9.36 <plus-minus> 2.66. A siginificant positive correlation was recognized between the fluoride ion concentrations and the age. And there was the fluoride ion concentrations in a tendency to lower as the BMD values became higher. However, the corelation between the fluoride concentrations and BMD values was not significant. A study about many subjects is necess
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