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Assessment of embryotoxic potential of mixtures of environmental chemicals using rat embryo limb bud cell cultures

Research Project

Project/Area Number 06670394
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Hygiene
Research InstitutionNational Institute for Environmental Studies

Principal Investigator

YONEMOTO Junzo  National Institute for Environmental Studies, Regional Environment Senior Division Researcher, 地域環境研究グループ, 主任研究員 (30072664)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
KeywordsLimb bud cell cultures / Environmental chemicals / Embryotoxicity / Toxicity assessment of mixtures / Organochlorine compounds / Isobole diagram / 混合物の相互作用
Research Abstract

The objective of this study was to evaluate the usefulness of rat embryo limb bud cell cultures (LBC) for analyzing joint embryotoxic action of chemical contaminants in the environment. The mode of joint action of chemicals in LBC can be visualized using the toxic unit model and an isobole diagram.
The embryotoxicity of nine organochlorine compounds commonly detected in the environment were screened using LBC.These chemicals were p-dichlorobenzene (pDCB), o-dichlorobenzene (o-DCB), p-chloroaniline(pCA), 3,4-dichloroaniline (DCA), tris(2-chloroethyl) phosphate, 2,5-dichlorophenol (DP), 2,5-dichloroanisole (DA), Triclosan (IR) and Triclocarban (TC). The embryotoxicity of binary mixtures of these chemicals were also screened and analyzed using LBC and isobole diagram. Mixture of IR-TC showed additive joint action. Mixtures of pDCB-TCEP and pDCB-pCA showed less than additive joint action for cell proliferation while mixture of pDCB-DP showed less than additive joint action for cell differentiation.
As LBC can assess the joint action for cell proliferation, for cell differentiation and for their ratio of the former to the latter (P/D ratio), LBC is useful for evaluating embryotoxic potential of chemical mixtures.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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