| Project/Area Number |
06670448
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Legal medicine
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
SAGISAKA Kaoru Tohoku University, SCHOOL of MEDICAL FORENSIC MEDICINE,PROFESSOR, 医学部, 教授 (70006740)
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| Co-Investigator(Kenkyū-buntansha) |
NATA Masayuki Tohoku University SCHOOL of MEDICAL FORENSIC MEDICINE,ASSISTANT, 医学部, 助手 (70241627)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | MIXED STAINS / ABH BLOOD GROUP / ELISA / 混合斑痕 / ABH式血液型 / Mixedstain, / ABH blood group, |
|---|
| Research Abstract |
FOR ABH BLOODTYPING MIXED-STAIN COMSISTING OF THE SAME KIND OF BODY FLUIDS,WE TRYED TO SPECIFIC ABSORBE A OR B BLOOD EPITOPE BY NITROCELLULOSE OR MICROTITE PLATE WHICH WAS COATED WITH ANTI-A OR-B ANTIBOY.HOWEVER,WE COULD NOT ABSORBE A OR B EPITOPE DUE TO LOW CAPACITY OF THE MEMBRANE AND PLATE.TO SEPARATE ABH EPITOPES OF MIXED STAIN SCOMSISTING OF DIFFERENT KIND OF BODY FLUIDS,WETTIED TO PREPARE MONOCLONAL ANTLIBODY AGAINST TEAR OR AMNITIC FLUID.IT WAS REVEALED THAT BOTH THE ANTIBOIES HAD SPECIFICITY TO PROTEINS IN TEAR OR AMNITIC FLUID carrying ABH blood group epitopes. IT IS CONSIDERED THAT THE MONOCLONAL ANTIBOIES COULD BE APLLYED TO FORENSIC PRACTICE TO DETECT SEPARATELY ABH BLOOD GROUP FROM MIXED STAINS CONSISTING OF DIFFERENT KIND OF BODY FLUID.
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