| Project/Area Number |
06670457
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Legal medicine
|
|---|
| Research Institution | SHIMANE MEDICAL UNIVERSITY |
|---|
Principal Investigator |
KIMURA Kojiro Shimane Medical Univ., Legal Med., Professor, 医学部, 教授 (30153191)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
IDZU Tomoko Shimane Medical Univ., Legal Med., Assistant, 医学部, 助手 (80263515)
TAKAHASHI Setsunori Shimane Medical Univ., Legal Med., Assistant, 医学部, 助手 (90032226)
MATSUBARA Kazuo Shimane Medical Univ., Legal Med., Associated professor, 医学部, 助教授 (20127533)
|
|---|
| Project Period (FY) |
1994 – 1995
|
| Project Status |
Completed (Fiscal Year 1995)
|
| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
|
|---|
| Keywords | Forensic toxicology / Kerosene / Gas chromatography / mass spectrometry / Trimethylbenzene / Body tissue |
|---|
| Research Abstract |
1) Determination of kerosene components
A sensitive and rapid method to analyze kerosene components was developed by making use of capillary gas chromatography/mass spectrometry (GC/MS) combined with the solvent extraction method using n-pentane.
The aliphatic hydrocarbons with carbon number over 9 and aromatics such as toluene, xylene and trimethylbenzenes were clearly detected in body tissues exposed to kerosene vapor. The lower limit of detection was ca. 10 pg in each of trimetylbenzenes.
These result suggest that this method is useful to detect fuel components in body tissues.
2) Relationship between the levels of kerosene components in body tissues and the concentration of kerosene vapor inhaled
Rats were exposed to kerosene vapor (1,000 ppm) in a chamber made by using common glass bottles and tubes for 5 or 15 min. Trimethylbenzenes including cumen, puseudocumene, mesitylene and 1,2,3-trimethylbenzene in tissues were selected as indicators to presume the concentration of kerosene vapor exposed. Blood levels of the four compounds increased in proportion to the inhalation time of kerosene vapor, and almost the same tendency was observed in the other body tissues. The concentrations of trimethylbenzenes in the lung were nearly the same as those in the blood either for 5 or 15 min-exposure to kerosene, so that the lung would be the most substitutive sample when blood could not be collected due to decomposition.
|
