| Project/Area Number |
06670464
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Legal medicine
|
|---|
| Research Institution | SAPPORO MEDICAL UNIVERSITY |
|---|
Principal Investigator |
MORITA Masahiko Sapporo Medical University, Dept.Legal Medicine Professor, 医学部, 教授 (00045353)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HATA Katsuhiko Sapporo Medical University, Dept.Legal Medicine Instructor, 医学部, 助手 (10228463)
AZUMI Jun-ichi Sapporo Medical University, Dept.Legal Medicine Assistant Professor, 医学部, 講師 (00045551)
FUNAYAMA Masato Sapporo Medical University, Dept.Legal Medicine Associate Professor, 医学部, 助教授 (40190128)
|
|---|
| Project Period (FY) |
1994 – 1995
|
| Project Status |
Completed (Fiscal Year 1995)
|
| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
|
|---|
| Keywords | Proliferating cell nuclear antigen / Immunohistochemistry / Wound healing / Rat skin / 増殖細胞マーカー |
|---|
| Research Abstract |
Wound healing accompanies cell proliferation. Recently, there are many reports that proliferating cell nuclear antigen (PCNA) is useful as the indications of cell division. In this paper, incised skin wounds of rats were studied by immunohistochemistry using monoclonal antibody against PCNA.As the great majority of epidermal basal cells (nuclei) of uninjured rat skin showed PCNA positive reaction, it is difficult to differentiate between antemortem and postmortem wounds. The authors observed the distribution of PCNA positive cells around wound area during wound healing. A few of PCNA positive cells appeared on the surface of 6-hr vital wounds. In oneday-old skin lesion, a number of fibroblasts and endothelial cells of capillaries stained positive. The number of positive cells reached a peak in about 4 days after wounding. Though there were still a good many of cells in granulation tissue with a wound age of 5-6 days, the number of PCNA negative cells increased. The relative decrease of PCNA positive cells seemed to be associated with the increase of collagen. Using PCNA staining, it can be easily followed the beginning of cell division on fresh wound surface and the highest peak of it in granulation.
|
