Effects of anoxia or hypoxia on morphological changes and leakage of cytoplasmic substances in cultured adult rat cardiomyocytes.
| Project/Area Number |
06670465
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Legal medicine
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| Research Institution | Nagoya City University |
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Principal Investigator |
MATOBA Ryoji Nagoya City University, Medical School, Professor, 医学部, 教授 (20107056)
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| Co-Investigator(Kenkyū-buntansha) |
MAENO Yoshitaka Nagoya City University, Medical School, Lecturer, 医学部, 講師 (00145749)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Anoxia / Ischemic heart disease / Adult rat / Cardiomyocyte / CPK / DNA / Necrosis / Apoptosis / 酸欠 / 培養細胞 / 心室筋細胞 / LDH / CPN / 単離心筋細胞 / Caイオン |
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| Research Abstract |
To establish the diagnosis of ischemic heart disease in the early atage, we studied whether cell injuries including cell death were led in vitro during anoxia (or hypoxia) using cultured adult rat cardiomyocytes. In the hypoxic condition of 20 mmHg PO2, morphological changes such as cellular contraction, swelling, disappearance of cross striations and bleb formation were observed up to 4 hr. Creatine phosphokinase contents in the extracellular medium also increased. Moreover, longer period of hypoxic incubation resulted in DNA degradation including DNA fragmentations with 180 bp multiples. These results suggest that these morphological changes and apoptosis in forensic pathological materials may be useful for the diagnosis of ischemic heart disease.
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Report
(3 results)
Research Products
(6 results)
