Estimation of vital reaction using interleukin 1 in human skin wounds

Research Project

Project/Area Number	06670466
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Legal medicine
Research Institution	Nagoya City University
Principal Investigator	MAENO Yoshitaka Nagoya City University, Medical School, Lecturer, 医学部, 講師 (00145749)
Co-Investigator(Kenkyū-buntansha)	INOUE Hiroyuki Nagoya City University, Medical School, Assistant, 医学部, 助手 (40159992)
Project Period (FY)	1994 – 1995
Project Status	Completed (Fiscal Year 1995)
Budget Amount *help	¥400,000 (Direct Cost: ¥400,000) Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Keywords	Vital reaction / Human skin / Wounds / Interleukin-1 / Immunohistochemistry / サイトカイン / IL-1α / IL-1β
Research Abstract	It is most important to estimate the vital reaction of wounds with short survival times. Interleukin-1 (IL-1) is produced constitutively and in response to injury and infection by a number of cell types. Therefore we investigated whether IL-1 expressed in human skin wounds and it could be useful for the estimation of wound age. Human skin wounds were obtained from forensic autopsies. After fixed in 10% buffered formalin, skins were embedded in paraffin. The paraffin-embedded specimens were sliced. Slices were stained with HE and were immunostained with anti human IL-1alpha or beta antibody. The IL-1beta expression was seen in lymph, macrophage or fibroblasts in 12 hr-old wound and 1 week-old wounds whereas it was not observed in wounds with very short survival times. These results suggest that IL-1 can be useful for estimating a long term of wound age in human cases.