| Project/Area Number |
06670468
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Juntendo University |
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Principal Investigator |
KIMURA Hiroko Department of Forensic Medicine, School of Medicine, Lecturer, 医学部, 講師 (00053299)
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| Co-Investigator(Kenkyū-buntansha) |
ITOH Yukio Department of Forensic Medicine, School of Medicine, Lecturer, 医学部, 講師 (70053345)
北森 武彦 東京大学, 工学部, 助教授 (60214821)
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| Project Period (FY) |
1994 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Photothermal spectroscopy / Immunoassay / Colloidal gold / Laser / Capillary zone electrophoresis / CEA / Drug / Lawis抗原 / 顕微熱レンズ分光法 / リンパ球 / 免疫測定 / レーザー光熱偏向分光法 / ラテックス粒子 / carillary zone electrophoresis / ハプテン / capillary zone electrophoresis / ABO式血液型抗原 / Lewis式血液型抗原 / 薬毒物 |
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| Research Abstract |
We developed several new immunoassays based on highly sensitive detection by a photo-thermal conversion system, in place of conventional absorption spectrophotometric detectors. They are an immunobinding assay using photothermal deflection spectroscopy, a capillary zone electrophoresis (CZE) which uses capillary vibration induced by laser (CVL) and a photothermal microscopy. The new feature of these methods is the use of colloidal gold which is feasible to laser photothermal method as a labeling material.
Using these newly developed immunoassay methods, we could detect and quantitate trace antigens in human sera, i.e.carcinoembryonic antigen, IgE,Lewis antigens. We could also detect human chorionic gonadotropin, and some drugs, methamphetamine in urine and antibiotics in a epithelial cell of renal tubules.
These assay methods are sensitive and easy enough to detect trace substances in samples. Therefore, these new immunoassays are applicable to forensic medical test and/or clinical diagnosis.
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