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PROTEIN KINASE ISOFORM ISOFORMS IN LYMPHOID CELLS.

Research Project

Project/Area Number 06670476
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionHokkaido University

Principal Investigator

TSUTSUMI Akito  Hokkaido Univ. School of Medicine. Instructor in Medicine., 医学部附属病院, 助手 (60250453)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsProtein Kinase C / Isoform / Lymphocytes / PKCdelta / Somatostatin receptors
Research Abstract

1) A Western blot system for detecting PKC isoforms in lymphoid cells was developed.
2) Polymerase chain reaction (PCR) primers for PKC lambda rheta and eta were made. Reverse transcription-PCR (RT-PCR) with a mouse preB cell line 70z/3 and a mouse B cell line WEHI231 showed that PKC lambda and rheta mRNA is detectable in these cell lines.
3) An expression vector for overexpression of PKCdelta was constructed using PKCdelta cDNA.The vector was transfected to 70z/3 cells and Jurkat human lymphoid cells. Unexpectedly, we could not detect overexpression of PKCdelta protein by Western blot analysis. The reason for this is now under investigation.
4) Using the same methodology, somatostatin receptor (SSTR) subtype expression in human lymphoid cells was investigated. It was found that although there is heterogeneity in SSTR subtypes in lymphoid cells, SSTR2 is the most dominant subtype. It was also found that although human peripheral lymphoid cells express little or no SSTR2 mRNA,peripheral lymphocytes from patients with lymphatic leukemia may express abundant amount of SSTR2 mRNA.These results suggest that expression of SSTR2 may have a role in development, activation and tumorgenesis of human lymphoid cells.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Tsutsumi A: "Antibodies to β2-Glycoprotein I and clinical manifestations in patients with systemic lupus erythematosus." Arthritis Rheum, in press.(1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koike T: "Antigenic specificity of the “anticardiolipin" antibodies." Blood. 85. 2277-2278 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Amasaki Y: "Up-regulated expression of Fas antigen (CD95) by peripheral naive and memory T cell subsets in patients with systemic lupus erythematosus (SLE): a possible mechanism for lymphopenia." Clin Exp Immunol. 99. 245-250 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsuboi A: "Calcineurin activates transcription from the GM-CSF promoter in synergy with either protein kinase C or NF-κB/AP-1 in T cells." Biochem Biophys Res Comm. 199. 1064-1072 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koike T: "Pulmonary hypertension and the antiphospholipid syndrome." Internal Medicine. 34. 938 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsutsumi A: "Pathogenic anticardiolipin antibodies in patients with SLE recognize epitopes expressed on beta2-Glycoprotein I." Lupus. 4(2). 42 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsutsumi A: "Lymphocyte subsets express different amounts of PKC isoform proteins." J Cell Biochem. suppl 18D. 88 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsutsumi A et al.: "Antibodies to beta2-Glycoprotein I and clinical manifestations in patients with systemic lupus erythematosus." Arthritis Rheum. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koike T,Tsutsumi A et al.: "Antigenic specificity of the "anticardiolipin" antibodies." Blood. 85. 2277-2278 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Amasaki Y,Kobayashi S,Takeda T,Ogura N,Jodo S,Nakabayashi T,Tsutsumi A et al.: "Up-regulated expression of Fas antigen (CD95) by peripheral naive and memory T cell subsets in patients with systemic lupus erythematosus (SLE) : a possible mechanism for lymphopenia." Clin Exp Immunol. 99. 245-250 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsuboi A Muramatsu M,Tsutsumi A et al.: "Calcineurin activates transcription from the GM-CSF promoter in synergy with either protein kinase C or NF-kappaB/AP-1 in T cells." Biochem Biophys Res Comm.199. 1064-1072 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koike T,and Tsutsumi A.: "Pulmonary hypertension and the antiphospholipid syndrome." Internal Medicine. 34. 938 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsutsumi A et al.: "Pathogenic anticardiolipin antibodies in patients with SLE recognize epitopes expressed on beta2-Glycoprotein I." Lupus. vo.4 suppl.2. 42 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsutsumi A et al.: "Lymphocyte subsets express different amounts of PKC isform proteins." J Cell Biochem. suppl 18D. 88 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koike T: "Antigenic specificity of the“anticardiolipin"antibodies." Blood. 85. 2277-2278 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Amasaki Y: "Up-regulated expression of Fas antigen(CD95)by peripheral naive and memory T cell subsets in patients With systemic lupus erythematosus(SLE):a possible mechanism for lymphopenia." Clin Exp Immunol. 99. 245-250 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tsuboi A: "Calcineurin activates transcription from the GM-CSF promoter in synergy with either protein kinase C or NF-κB/AP-1 in T cells." Biochem Biophys Res Comm. 199. 1064-1072 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tsutsumi A: "Pathogenic anticardiolipin antibodies in patients with SLE recognize epitopes expressed on beta2-Glycoprotein I." Lupus. 4. 42- (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tsutsumi A: "Lymphocyte subsets express different amounts of PKC isoform proteins." J Cell Biochem. suppl 18D. 88- (1994)

    • Related Report
      1995 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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